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| Anti-Fibroblast MicroBeads |
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| Overview |
Anti-Fibroblast MicroBeads have been developed for the positive selection or depletion of human fibroblasts. Applications of the Anti-Fibroblast MicroBeads include:
- depletion of human fibroblasts that are used as feeder cells to support the growth of ES or iPS cells,
- isolation of MSCs from bone marrow aspirate,
- removal of fibroblastic cells from bone marrow cultures or other primary tissue cultures,
- positive selection of fibroblasts from a mixture of cells,
- depletion of fibroblast cells during establishment of primary tumor cell lines in order to prevent fibroblastic overgrowth within the tissue culture.
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| Details |
Background information
Anti-Fibroblast MicroBeads are based on a fibroblast-specific antigen.1 This antigen is also known to be expressed on mesenchymal stem cells, also known as mesenchymal stromal cells, (MSCs) in the bone marrow.2–4 |
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| Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator. |
| Before separation |
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| After depletion |
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| Isolated fibroblasts |
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| Favorites / Prices |
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| Details |
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| Products |
| Anti-Fibroblast MicroBeads, human |
- for 109 total cells
Download datasheet
130-050-601
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| References |
| 1. Fearns and Dowdle (1992) Int. J. Cancer 50: 621–627. |
| 2. Jones et al. (2002) Arthritis and Rheumatism 46: 3349–3360. |
| 3. Jones et al. (2006) Clin. Cytom. 70: 391–399. |
| 4. Jones et al. (2007) MACS&more 11 (1): 22–25. |
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