Anti-Fibroblast MicroBeads
Description Anti-Fibroblast MicroBeads have been developed for the positive selection or depletion of human fibroblasts using a fibroblast-specific antigen.1 This antigen is also known to be expressed on mesenchymal stromal cells (MSCs) in the bone marrow.2 Applications Applications of the Anti-Fibroblast MicroBeads include the isolation of MSCs from bone marrow aspirate or the removal of fibroblastic cells from bone marrow cultures or other primary tissue cultures. The depletion of fibroblast cells can be an effective step in the establishment of primary tumor cell lines in order to prevent fibroblastic overgrowth within the tissue culture. Furthermore, human fibroblasts that are used as feeder cells to support the growth of ESC or iPS cells can be depleted by using the Anti-Fibroblast MicroBeads. Columns For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.		Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator. Before separation After depletion Isolated fibroblasts

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