| Description |
Interleukin-13 (IL-13) is produced primarily by activated Th2 cells, but also by mast cells, NK cells, and dendritic cells. IL-13 exerts anti-inflammatory effects on monocytes and macrophages. Furthermore, it inhibits the expression of pro-inflammatory cytokines, such as IL-1β, TNF-α, IL-6, and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in an increased production of IgE.
IL-13, in contrast to IL-4, fails to induce Th2 cell differentiation, one of the hallmarks of the allergic response. |
| Applications |
| This Anti-IL-13 antibody has been developed for intracellular staining of IL-13 in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by in-column intracellular staining technology in combination with MACS® Separation. |
| Cross-reactivity |
| Rhesus monkey (Macaca mulatta) |
| Clone | Isotype |
| JES10-5A2.2 | Rat IgG1 |
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| Figure 1 |
| Human PBMCs were stimulated with CytoStim for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, and stained with Anti-IL-13-PE and CD154-APC. Cells were analyzed by flow cytometry. Plots show CD4+ lymphocytes gated according to CD4-FITC staining. |
| Stimulated sample |
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| Unstimulated control |
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