Anti-IFN-α
Description Type I interferons (IFNs), including IFN-α, are a family of cytokines that exert pleiotropic functions in the immune system. Besides its potent antiviral activity, IFN-α exerts a variety of immunomodulatory effects, including positive and negative regulation of its own production, regulation of IL-12 and IFN-γ production, promotion of natural killer (NK) cell cytotoxicity, activation and maturation of dendritic cells, and the promotion of B cell development towards plasma cells. IFN-α is expressed predominantly by CD123+CD11c-CD303 (BDCA2)+ plasmacytoid dendritic cells (pDCs). Applications The antibody has been developed for intracellular staining of IFN-α in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Cell Separation. Clone Isotype LT27:295 Mouse IgG1		Figure 1 Human PBMCs were incubated with or without CpG ODN 2216 for 6 hours. After 3 hours, brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-α-PE. Cells were analyzed by flow cytometry. A: Stimulated sample B: Unstimulated control