CD154/IL-5/CD4 Detection Kit
Description The CD154/IL-5/CD4 Detection Kit is designed for the intracellular detection of IL-5 production in activated CD4+ T cells. It contains an antibody recognizing CD154 – a specific marker for T cell activation – and all reagents for the immunofluorescent detection of IL-5-producing activated CD4+ T cells from whole blood or PBMCs. By combining the detection of cytokine production with staining of CD154 and CD4, the sensitive and specific detection of activated antigen-specific CD4+ T cells is easily possible. IL-5 is predominantly secreted by CD4+ T cells. It is involved in a range of allergic reactions and mediates immune responses to parasites. IL-5 also acts on other cell types such as B cells. Applications The kit is suited for flow cytometric identification and enumeration of IL-5-producing CD154+CD4+ T cells upon in vitro stimulation. Other applications include identification and enumeration of IL-5-producing, antigen-specific CD154+CD4+ T cells upon restimulation or monitoring of specificity of antigen-specific T cell lines. Clone Isotype Anti-IL-5: JES1-39D10 Rat IgG2a CD154: 5C8 Mouse IgG2a CD4: VIT4 Mouse IgG2a		Figure 1 Human PBMCs were incubated with or without CytoStim for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with CD154-APC, Anti- IL-5-PE, and CD4-FITC. Cells were analyzed by flow cytometry. A: Stimulated sample B: Unstimulated control