IL-10 Secretion Assays - Detection Kits
Description Interleukin-10 (IL-10) is predominantly secreted by human CD4+ T cells and antigen-presenting cells, such as monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by TH1 cells, activated monocytes, and NK cells. It can stimulate the immunoglobulin production by B cells. The IL-10 Secretion Assays enable the sensitive detection of human IL-10-secreting cells. Applications IL-10 Secretion Assays were used to analyze IL-10-secreting, CMV-specific CD4+ T cells after stimulation with CMV lysate. The IL-10 Secretion Assay was also used to analyze and isolate IL-10 secretion by allergen-specific T cells.1,2 The IL-10 Secretion Assay - Detection Kits can be used for two-color cytokine analysis. Further information Detection of cytokine secreting cells from whole blood [PDF; 70,5 KB] Two-color Cytokine Secretion Assays [PDF; 98,3 KB] Combined staining of cytokine-secreting cells with peptide-MHC tetramers [PDF; 101,8 KB] Detection of cytokine secreting cells [PDF; 165,1 KB]		Figure 1 PBMCs from a CMV+ donor were stimulated with CMV-lysate. The responding cells were stained for co-expression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4+ T cells. A: Stimulated sample B: Unstimulated control * Percentage represents frequency among CD4+ T cells.