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| IL-17 Secretion Assay – Detection Kits |
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| Description |
IL-17 is produced by CD4+ T helper cells, a T cell subset termed Th17, which also secrete IL-17F and IL-22 and express the NK cell marker CD1611. IL-17 secretion has also been described for other cell types, such as CD8+ memory T cells.
Th17 cells are involved in the recruitment of neutrophils to control early stages of infections by a number of pathogens, such as extracellular bacteria and fungi. IL-17 and Th17 cells play an important role in immune-mediated inflammatory diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, and inflammatory bowel disease.
The IL-17 Secretion Assay – Detection Kit allows the detection of viable human IL-17–secreting leukocytes from PBMCs. |
| Applications |
| Detection of viable IL-17–secreting leukocytes from whole blood, PBMCs, or other leukocyte-containing single-cell preparations. |
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| Figure 1 |
| PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, stimulated and unstimulated cells were analyzed by using the IL-17 Secretion Assay – Detection Kit (PE) or (APC). Secretion of IL-17 by CD154+ cells is demonstrated. Data were gated on viable CD4+ cells. |
| IL-17 Secretion Assay - Detection Kit (PE) |
| A: Restimulated sample |
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| B: Unstimulated control |
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| IL-17 Secretion Assay - Detection Kit (APC) |
| A: Restimulated sample |
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| B: Unstimulated control |
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| References |
| 1. Cosmi et al. (2008) J. Exp. Med. 205: 1903–1916. |
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