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| Description |
| The CD146 MicroBead Kit was developed for the isolation of CD146+ cells, such as human endothelial cells, umbilical vein endothelial cells (HUVECs), and pericytes from human tissue. The kit consists of FcR Blocking Reagent and CD146 MicroBeads that recognize the human CD146 antigen, also known as MUC18, MCAM, Mel-CAM, and S-Endo-1. |
| Applications |
CD146 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and contains five extracellular immunoglobulin (Ig)-like domains.1, 2 CD146 possesses a limited tissue expression distribution, including endothelial cells, smooth muscle cells, pericytes, follicular dendritic cells, melanoma cells, and a sub-population of activated T lymphocytes.1, 3
CD146 functions as a Ca2+-independent cell adhesion molecule which is involved in heterophilic cell-cell interactions4, and may be involved in the extravasion and/or homing of activated T cells3. CD146 expression in melanoma is also directly associated with tumor growth and metastasis.5
The CD146 MicroBead Kit can be used for:
- Isolation of endothelial cells and pericytes from human endothelial tissue.
- Isolation of human umbilical vein endothelial cells (HUVECs) and human umbilical cord perivascular cells (HUCPVCs)4 from umbilical cord vein.
- Isolation of mesenchymal stromal cells (MSCs) from human tissues, such as lipoaspirate.
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| Columns |
| For positive selection: LS, or XS Columns. |
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| Figure 1 |
| CD146+ cells were separated from lipoaspirate using the CD146 MicroBead Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD146-APC and CD34-FITC. |
| Before separation |
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| CD146– cell fraction |
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| CD146+ cell fraction |
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| Details |
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| References |
| 1. Bardin et al. (2001) Blood 98: 3677–3684. |
| 2. Yan et al. (2003) Blood 102: 184–191. |
| 3. Pickl et al. (1997) J. Immunol. 158: 2107–2115. |
| 4. Xie (1997) Cancer Res. 57: 2295–2303. |
| 5. Satyamoorthy et al. (2001) Oncogene 20: 4676–4684. |
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