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| Overview |
CD105 MicroBeads are developed for the isolation of CD105+ endothelial cells from single-cell suspensions of endothelial tissues, as well as for the isolation of mesenchymal stromal cells (MSCs) from bone marrow aspirate.
CD105 MicroBeads can, for example, be used for the isolation of endothelial cells from:
- umbilical cord veins,
- arterial vessels1,
- tumors2,
- cultures of endothelial cells that are derived from synovial tissue.3
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| Details |
Background information
The CD105 antigen (endoglin) serves as a receptor for the growth and differentiation factors TGF-β1 and TGF-β3, and is expressed on mature endothelial cells, on MSCs with mesodermal differentiation capacity, and on some leukemic cells of B lymphoid and myeloid origin. The CD105 MicroBeads recognize the same molecular antigen as anti-SH2 antibodies-the most commonly employed antigen for the definition of cultivated MSCs.
Downstream applications
CD105 MicroBeads can be used for studies on mesengenesis and for in vitro investigations on hematological disorders.4 CD105+ bone marrow cells also show multipotent differentiation in vitro5 and the capacity to form bone in vivo without prior cultivation or differentiation6. |
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| Figure 1 |
| Separation of CD105+ endothelial cells from a single-cell preparation of umbilical cord vein tissue using CD105 MicroBeads, an MS Column, and a MiniMACS™ Separator. |
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| Figure 2 |
| CD105+ cells isolated from umbilical cord vein endothelium. |
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| Figure 3 |
| Immunomagnetically selected CD105+ cells after 25 days in culture. Cells were isolated from the bone marrow aspirate of an acute myeloid leukemia patient.4 |
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| Details |
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| References |
| 1. Dentelli et al. (2007) Blood 109: 4264–4271. |
| 2. Fonsato et al. (2006) Am. J. Pathol. 168: 706–713. |
| 3. Santiago et al. (2006) Arthritis Res. Ther. 8: R43. |
| 4. Campioni et al. (2003) MACS&more 7 (1): 11-13. |
| 5. Majumdar et al. (2000) J. Cell. Physiol. 185: 98–106. |
| 6. Aslan et al. (2006) Stem Cells 24: 1728–1737. |
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