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| Overview |
| The CD31 MicroBead Kit has been developed for the isolation of CD31+ endothelial cells from single-cell suspensions. |
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Background information
CD31 is a glycoprotein, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1). It is expressed on mature endothelial cells, platelets, and on some white blood cells such as monocytes, NK cells, granulocytes, B cells, and T cell subsets.
Applications
The CD31 MicroBead Kit can be used for the isolation of human dermal vascular endothelial cells (HDMECs) from dermal tissues, for example foreskin, and human umbilical vein endothelial cells (HUVECs) from umbilical cord vein.
The CD31 MicroBead Kit has also been used for:
- the isolation of endothelial cells from colorectal cancer and healthy colon tissue1,
- the enrichment of lymphangioma-derived lymphatic endothelial cells from fibroblast-contaminated cultures2,
- the isolation of human microvascular endothelial cells from dermal or renal tissue3.
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| Figure 1 |
| Separation of CD31+ HDMECs from a preparation of dermal-layer cells of human foreskin using the CD31 MicroBead Kit and a MidiMACS™ Separator with an LS Column. Cells were fluorescently stained with CD31-APC. |
| A: HDMECs before separation |
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| B: CD31– cells |
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| C: CD31+ cells |
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| Figure 2 |
| Cultured HDMECs after extraction from foreskin tissue and purification using the CD31 MicroBead Kit. |
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| References |
| 1. Schellerer et al. (2007) Lab. Invest. 87: 1159–1170. |
| 2. Norgall et al. (2007) BMC Cancer 7: 105. |
| 3. Fonsato et al. (2006) Am. J. Pathol. 168: 706–713. |
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