| Description |
Microglia, often called brain macrophages, are the resident immune-effector cells in the CNS. In addition, activated microglia serve as the major antigen-presenting cells in the CNS1. They are morphologically, immunophenotypically, and functionally related to cells of the monocyte/macrophage lineage2.
The most characteristic feature of microglial cells is their rapid activation in response to injury, inflammation, neurodegeneration, infection, and brain tumors. A paucity of microglial antigens has hindered microglial identification.
However, CD11b has been widely used as marker for microglial identification and is also suitable for their immunomagnetic isolation from mouse brain tissue3-6. |
| Applications |
| CD11b (Microglia) MicroBeads have been used to isolate CD11b+ cells from single cell suspensions of brain tissue3-6. |
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| Figure 1 |
| Whole-brain tissue from P1 mice was first dissociated to single cells using the Neural Tissue Dissociation Kit (T) and then microglia were isolated using CD11b (Microglia) MicroBeads. |
Before separation
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| Microglia CD11b+ fraction |
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