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| Description |
Anti-PSA-NCAM MicroBeads have been developed for the isolation of PSA-NCAM+ cells. Anti-PSA-NCAM MicroBeads recognize polysialic acid (PSA) which, in vertebrates, is linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56)1. PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed in embryonic and neonatal neural tissue2. In adult mammalian brain PSA-NCAM expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ)3 and the dentate gyrus of the hippocampus4.
PSA-NCAM is a marker for immature neuronal-committed progenitors that are permanently generated in the SVZ and migrate along a well-defined pathway, the rostral migratory stream, into the olfactory bulb where they differentiate into GABAergic and dopaminergic interneurons3,5.
Antibodies against PSA-NCAM have been used to immunomagnetically isolate neuronal progenitors from postnatal mice and rat forebrain.6–10 |
| Applications |
Positive selection or depletion of human, mouse, or rat cells expressing PSA-NCAM.
Positive selection of neuronal progenitor cells, e.g., from mouse SVZ tissue in conjunction with prior depletion of A2B5+ glial progenitor cells.6,7 |
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| Figure 1 |
| Separation of a single-cell suspension derived from P1 mouse whole-brain tissue using the Neural Tissue Dissociation Kit (T), Anti-PSA-NCAM MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with rat anti-mouse IgM-APC and analyzed by flow cytometry. |
| A: Neural cells before separation |
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| B: PSA-NCAM- cell fraction |
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| C: Isolated PSA-NCAM+ cells |
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| Products |
| Anti-PSA-NCAM MicroBeads, human, mouse, rat |
for 1x109 total cells
Download data sheet
130-092-966
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| MACS References |
| 1. Rougon et al (1986) J. Biol. Chem. 261: 3396-3401 |
| 2. Kiss et al (2001) Rev. Neurosci. 12: 297-310 |
| 3. Doetsch et al (1997) J. Neurosci 17: 5046-5061 |
| 4. Seki (2002) J. Neurosci. Res. 70: 327-334 |
| 5. Pennartz et al (2004) Mol. Cell. Neurosci. 25: 692-706 |
| 6. Seidenfaden, R. et al. (2006) Mol. Cell. Neurosci. 32: 187–98.[8815] |
| 7. Seidenfaden, R. et al. (2006) MACS&more 10 (1): 4–6. |
| 8. Marmur, R. et al. (1998) J. Neurosci. 18: 9800–9811.[1252] |
| 9. Strathmann et al (2007) BMC Dev. Biol 7:33 |
| 10. Widera, D. et al. (2006) BMC Neuroscience. 7:64, 1-18 |
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