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| Neural Tissue Dissociation Kits |
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| Overview |
The Neural Tissue Dissociation Kits have been developed for the gentle and efficient generation of single-cell suspensions from neural tissues.
Depending on the application the kits and can be used with manual mechanical dissociation or with automated dissociation, namely the gentleMACS™ Dissociator and the gentleMACS Octo Dissociator.
The Neural Tissue Dissociation Kit - Postnatal Neurons has been optimized for the special needs of sensitive postnatal neurons during dissociation. |
| Details |
Background information
In less than an hour, the gentle two-step enzymatic dissociation procedure efficiently yields high numbers of viable cells. The Neural Tissue Dissociation Kit (P) contains papain, and the Neural Tissue Dissociation Kit (T) contains trypsin.
Some antigen epitopes are damaged by papain, others by trypsin. The kit based on papain is best suited for use with Anti-Prominin-1 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, Anti-GLAST (ACSA-1) MicroBead Kit and Anti-PSA-NCAM MicroBeads require the use of the trypsin-based kit.
Please see figure 1 for further information with regard to epitope sensitivity and kit selection. |
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| Figure 1 |
Antigen compatibility of Neural Tissue Dissociation Kits.
The table shows the recommended kit regarding antigen compatibility for subsequent MACS® Cell Separation. Epitope sensitivities have been tested for antibodies from Miltenyi Biotec if available. |
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| Details |
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| References |
| 1. Környei et al. (2007) FASEB J. 21: 2496–2509. |
| 2. Lee et al. (2008) J. Neurosci 28: 8517 - 8528. |
| 3. Skog et al. (2008) Nat Cell Biol 10(12): 1470-6. |
| 4. Szulwach et al. (2010) J. Cell Biol. 189: 127 - 141. |
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