T Cell Activation/Expansion Kit, non-human primate
Description The T Cell Activation/Expansion Kit is designed to activate and, if required, expand rhesus monkey T cells. The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against non-human primate CD3, and human CD2 and CD28. Anti-Biotin MACSiBead™ Particles loaded with biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells from PBMCs as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 14 of culture. Applications Activated T cells can be used for many downstream applications, such as cytokine analysis or immunoprecipitation, and cells can also be transfected with high efficiency.		Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-APC and CD25-PE, or CD4-APC and CD69-PE. T cells after activation Negative control

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