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| T Cell Activation/Expansion Kit, non-human primate |
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| Description |
| The T Cell Activation/Expansion Kit was developed to activate and, if required, expand rhesus monkey T cells. |
| Details |
Background information
The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against non-human primate CD3, and human CD2 and CD28. Anti-Biotin MACSiBead™ Particles loaded with biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells from PBMCs as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 14 of culture.
Downstream applications
Activated T cells can be used for many downstream applications, such as cytokine analysis or immunoprecipitation, and cells can also be transfected with high efficiency. |
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| Figure 1 |
| Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-APC and CD25-PE, or CD4-APC and CD69-PE. |
| T cells after activation |
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| Negative control |
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| Details |
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| Products |
| T Cell Activation/Expansion Kit, non-human primate |
Components
- 2 mL Anti-Biotin MACSiBead™ Particles, cell culture grade
- 0.4 mL CD2-Biotin, human
- 0.4 mL CD3-Biotin, non-human primate
- 0.4 mL CD28-Biotin, human
Download datasheet
130-092-919
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