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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD235a (Glycophorin A) MicroBeads
Description
CD235a (Glycophorin A) MicroBeads can be used for the positive selection or depletion of human erythroid cells. The CD235a antigen (glycophorin A), a single-pass transmembrane glycoprotein, is expressed on mature erythrocytes and erythroid precursor cells.
Applications
Example applications include the enrichment of pluripotent stem cells from human bone marrow aspirate by a depletion strategy using CD235a (Glycophorin A) MicroBeads together with CD45 MicroBeads.1
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns.
For depletion: LD, CS, D, or autoMACS® Columns.
 
Figure 1
Separation of CD235a+ cells from cord blood mononuclear cells using CD235a (Glycophorin A) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD235a-FITC antibody and CD71-PE.
Cord blood mononuclear cells before separation
CD235a- cells
Isolated CD235a+ cells
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Details
Products
CD235a (Glycophorin A) MicroBeads, human
- for 109 total cells
Download datasheet
130-050-501
Qty.:
 

Related products
CD71 Antibodies
CD71 MicroBeads, human (#130-046-201)
CD45 MicroBeads, human (#130-045-801)
References
1. Reyes et al. (2001) Blood 98: 2615–2625.
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