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| Overview |
| CD71 MicroBeads are developed for the positive selection or depletion of CD71-expressing cells from peripheral blood mononuclear cells (PBMCs), cord blood, bone marrow, or lymphoid tissue. |
| Details |
Background information
The CD71 antigen is also known as the transferrin receptor, which is essential for iron transport into proliferating cells. It is expressed on mesenchymal stromal cells (MSCs) in bone marrow1, on activated T and B lymphocytes, macrophages, and on all proliferating cells. It is up-regulated on lymphocytes during proliferative responses to antigens or mitogens but is not expressed on resting lymphocytes. Furthermore, CD71 is present on reticulocytes and erythroid progenitors in fetal liver, cord blood, and peripheral blood, but is downregulated as these cells differentiate into mature erythrocytes 2–4.
Downstream applications
- Enrichment of fetal early erythroid cells from maternal peripheral blood5,6
- Isolation of hematopoietic precursor cells committed to the erythroid lineage (BFU-E) when used in combination with the CD34 MultiSort Kit
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| Figure 1 |
| Isolation of CD71+ cells from maternal blood after depletion of leukocytes and monocytes using CD45 and CD14 MicroBeads. (Courtesy of Dr. Jürgen Büsch, Cologne, Germany.) |
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| Figure 2 |
| Separation of CD71+ cells from cord blood mononuclear cells (CB-MNCs) using CD71 MicroBeads and the Possel separation program on the autoMACS® Separator. Cells were fluorescently stained with CD71-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
| CB-MNCs before separation |
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| CD71+cells (reticulocytes) |
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| Figure 3 |
| Giemsa-stained reticulocytes isolated from human buffy coat using CD71 MicroBeads. |
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| Details |
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| References |
| 1. Pittenger, M.F. et al. (1999) Science 284: 143–147. |
| 2. Judd et al. (1980) J. Exp. Med. 152: 1430–1435. |
| 3. Loken et al. (1987) Blood 69: 255–263. |
| 4. Phillips et al. (1984) J. Exp. Med. 159: 993–1008. |
| 5. Büsch et al.(1994) Prenatal Diagn. 14: 1129–1140. |
| 6. Cheung et al. (1996) Nat. Genet. 14: 264–268. |
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