| Description |
| CD71 MicroBeads are developed for positive selection or depletion of CD71-expressing cells. The CD71 antigen is also known as the transferrin receptor, which is essential for iron transport into proliferating cells, and is expressed on marrow stromal cells (MSCs) from bone marrow1, activated T and B lymphocytes, macrophages, and on all proliferating cells. It is up-regulated on lymphocytes during proliferative responses to antigens or mitogens but is not expressed on resting lymphocytes. CD71 is present on reticulocytes and erythroid progenitors in fetal liver, cord blood, and peripheral blood, but is down-regulated as these cells differentiate into mature erythrocytes.2–4 |
| Applications |
| Fetal early erythroid cells have been enriched from maternal peripheral blood by using CD71 MicroBeads.5,6 Also, in combination with the MACS® CD34 MultiSort Kit, hematopoietic precursor cells committed to the erythroid lineage (BFU-E) can be isolated. |
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| Figure 1 |
| Isolation of CD71+ cells from maternal blood after depletion of leukocytes and monocytes using CD45 and CD14 MicroBeads. (Courtesy of Dr. JĂĽrgen BĂĽsch, Cologne, Germany.) |
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| Figure 2 |
| Separation of CD71+ cells from cord blood mononuclear cells (CB-MNCs) using CD71 MicroBeads and the Possel separation program on the autoMACS™ Separator. Cells were fluorescently stained with CD71-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
| CB-MNCs before separation |
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| CD71+cells (reticulocytes) |
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| Figure 3 |
| Giemsa-stained reticulocytes isolated from human buffy coat using CD71 MicroBeads. |
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