| Description |
| CD138 MicroBeads are designed for isolation or depletion of plasma cells from PBMCs, bone marrow, leukapheresis harvests, and single-cell suspensions from lymphoid tissues. The CD138 antigen, also known as syndecan-1, is primarily expressed on normal and malignant plasma cells in the bone marrow. It is further found on a subset of plasma cells in peripheral blood and some lymphoid tissues. The CD138 antigen is neither expressed on naive B cells, germinal center B cells, or memory B cells, nor on T cells or monocytes. For information on malignant plasma cells click here. |
| Applications |
| Since direct study of plasma cells is hampered by their low frequency, prior enrichment of plasma cells using CD138 MicroBeads allows more accurate cell analysis, e.g., by flow cytometry2. To ease the analysis of enriched plasma cells, CD138 MicroBeads are also available in combination with CD138-PE. Plasma cells purified with CD138 Microbeads are applied for molecular biological studies, such as PCR1,4,7,8, microarrays2,4,5,6, or protein analysis7. |
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| Figure 1 |
| CD138+ plasma cells were isolated from normal PBMCs using CD138 MicroBeads, an MS Column, and a MiniMACS™ Separator. |
| A: PBMCs before separation |
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| B: Enriched CD138+ cells |
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