|
|
 |
|
| Overview |
| CD138 MicroBeads were developed for the positive selection or depletion of plasma cells from PBMCs, bone marrow, leukapheresis harvests, and single-cell suspensions from lymphoid tissues. |
| Details |
Background information
The CD138 antigen, also known as syndecan-1, is primarily expressed on normal and malignant plasma cells in the bone marrow. It is further found on a subset of plasma cells in peripheral blood and certain lymphoid tissues. The CD138 antigen is neither expressed on naive B cells, germinal center B cells, memory B cells, nor on T cells or monocytes.
Downstream applications
Since the direct study of plasma cells is hampered by their low frequency, prior enrichment of plasma cells using CD138 MicroBeads allows more accurate cell analysis, e.g. by flow cytometry2. To ease the analysis of enriched plasma cells, CD138 MicroBeads are also available in combination with CD138-PE. Plasma cells purified with CD138 MicroBeads are applied for molecular biological studies, such as PCR1,4,7,8, microarrays2,4,5,6, or protein analysis7. |
| |
| Figure 1 |
| CD138+ plasma cells were isolated from normal PBMCs using CD138 MicroBeads, an MS Column, and a MiniMACS™ Separator. |
| A: PBMCs before separation |
 |
| B: Enriched CD138+ cells |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| Products |
| CD138 MicroBeads, human |
| For research use only |
- for 2×109 total cells
Download datasheet
130-051-301
|
| CD138 MicroBeads, human - lyophilized |
| For research use only |
- for 2x109 total cells
Download datasheet
130-097-614
|
| CD138 MicroBeads + CD138-PE, human |
| For research use only |
- for 2×109 total cells
Download datasheet
130-090-503
|
|
| References |
| 1. Draube et al. (2001) Ann. Hematol. 80: 83-89. |
| 2. Shaughnessy et al. (2001) Blood 98: 217-223. |
| 3. Horst et al. (2002) Clin. Exp. Immunol. 130: 370-378. |
| 4. Davies et al. (2003) Blood 102: 4504-4511. |
| 5. Tarte et al. (2003) Blood 102: 592-600. |
| 6. Zhan et al. (2003) Blood 101: 1128-1140. |
| 7. Kumar et al. (2004) Blood 104: 1159-1165. |
| 8. Lee et al. (2004) Blood 103: 2308-2315. |
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
