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| Overview |
| The CD38 MicroBead Kit was developed for the positive isolation or depletion of CD38+ cells from human PBMCs, bone marrow or tissue. The CD38+ cells are indirectly magnetically labeled with CD38-Biotin and Anti-Biotin MicroBeads. |
| Details |
Background information
CD38 is a 45 kDa multi-lineage type II transmembrane glycoprotein. It is expressed at varying levels on the majority of hematopoietic as well as on some nonhematopoietic cells. The expression level depends on the developmental state and the activation status. CD38 is absent on most primitive CD34+ stem cells and upregulated on CD34+ cells upon lymphocyte commitment.1
Applications
The CD38 MicroBead Kit can be applied for positive selection or depletion of hematopoietic cell subsets expressing human CD38 antigen, such as CD38+ B cells, T cells, and NK cells from PBMCs, bone marrow, or tissue. The kit is also suitable for the isolation of CD38+ plasma cells from tissue, such as bone marrow.
The CD38 MicroBead Kit can also be used for depletion of CD38+ cells to isolate CD38– cell subsets, e.g., CD34+ CD38– stem cells in combination with the CD34 MicroBead Kit. |
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| Figure 1 |
| Positive selection of CD38+ cells from PBMCs using the CD38 MicroBead Kit, an MS Column, and a MiniMACS Separator. CD38+ cells labeled with the CD38 MicroBead Kit were fluorescently stained with Anti-Biotin-FITC and CD19-PE. |
| Before separation |
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| Enriched CD38+ cells |
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| Figure 2 |
| Depletion of CD38+ cells from PBMCs using the CD38 MicroBead Kit, an LD Column, and a MidiMACS Separator. Cell fractions are fluorescently stained with Anti-Biotin-FITC and CD19-PE to detect cells labeled with the CD38 MicroBead Kit. |
| Before separation |
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| PBMCs depleted of CD38+ cells |
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| References |
| 1. Terstappen et al. (1991) Blood 77: 1218–1227. |
| 2. Deterre et al. (2000) Chem. Immunol. 75: 146–168. |
| 3. Sconocchia et al. (1999) Blood 94: 3864–3871. |
| 4. Terstappen et al. (1990) J. Leukocyte Biol. 48: 138–148. |
| 5. Takasawa et al. (1993) Science 259: 370–373. |
| 6. Howard et al. (1993) Science 262: 1056–1059. |
| 7. Dianzani et al. (1994) J. Immunol. 154: 952–959. |
| 8. Funaro et al. (1990) J. Immunol. 145: 2390–2396. |
| 9. Deaglio et al. (2002) Blood 99: 2490–2498. |
| 10. Zilber et al. (2000) Proc. Natl. Acad. Sci. USA. 97: 2840–2845. |
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