CD38 MicroBead Kit ( #130-092-263 )

CD38 MicroBead Kit

Description

The CD38 Microbead Kit is an indirect magnetic labeling system for isolation or depletion of CD38⁺ cells. The CD38⁺ cells are indirectly magnetically labeled with CD38-Biotin and Anti-Biotin MicroBeads.
CD38 is a 45 kDa multi-lineage type II transmembrane glycoprotein. It is expressed at varying levels on the majority of hematopoietic as well as on some nonhematopoietic cells. The expression level depends on the developmental state and the activation status. CD38 is absent on most primitive CD34⁺ stem cells and upregulated on CD34⁺ cells upon lymphocyte commitment.¹
To date, CD38 was shown to have three main functions. First, it acts as a bifunctional ectoenzyme catalyzing both the synthesis and hydrolysis of cyclic ADP–ribose, which itself participates in the regulation of cytoplasmic calcium levels.⁶ Second, CD38 is known to be a cell surface receptor taking part in lymphocyte adhesion to the endothelium via a CD38/CD31 interaction.⁷ Third, the CD38 receptor is involved in cell signaling processes that lead to the activation of T, B, and NK cells, as well as monocytes.^8–10 CD38 signaling requires a functional TCR/CD3 complex in T cells¹¹, a functional CD21/CD19 complex in B cells, and the presence of CD16 in NK cells.^{2, 8, 9}

Applications

The CD38 MicroBead Kit can be applied for positive selection or depletion of hematopoietic cell subsets expressing human CD38 antigen, such as CD38⁺ B cells, T cells, and NK cells from PBMCs, bone marrow, or tissue. The kit is also suitable for the isolation of CD38⁺ plasma cells from tissue, such as bone marrow.
Distinct CD38⁺ cell subsets can be isolated by sequential sorting, either in combination with a MACS® MultiSort Kit or after depletion of non-target cells.
The CD38 MicroBead Kit can also be used for depletion of CD38⁺ cells to isolate CD38^– cell subsets, e.g., CD34⁺CD38^– stem cells in combination with the the CD34 MicroBead Kit.

Columns

For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.

Figure 1

Positive selection of CD38⁺ cells from PBMCs using the CD38 MicroBead Kit, an MS Column, and a MiniMACS Separator. CD38⁺ cells labeled with the CD38 MicroBead Kit were fluorescently stained with Anti-Biotin-FITC and CD19-PE.

Positive selection

Before separation

Enriched CD38⁺ cells

Figure 2

Depletion of CD38⁺ cells from PBMCs using the CD38 MicroBead Kit, an LD Column, and a MidiMACS Separator. Cell fractions are fluorescently stained with Anti-Biotin-FITC and CD19-PE to detect cells labeled with the CD38 MicroBead Kit.

Depletion

Before separation

PBMCs depleted of CD38⁺ cells