| Description |
The Plasma Cell Isolation Kit was developed for the isolation of blood plasma cells according to the expression of the CD38 antigen. The kit contains all reagents necessary for performing two sequential magnetic separations: First, non-plasma cells are magnetically labeled and depleted by using a cocktail of biotin-conjugated CD2, CD3, CD14, CD15, CD22, CD34, CD56, CD123, and CD235a antibodies, as primary labeling reagent, and Anti-Biotin MicroBeads as secondary labeling reagent to be magnetically depleted. Then, the pre-enriched plasma cells in the non-magnetic flow-through fraction are specifically labeled with CD38 MicroBeads and isolated by positive selection.
Human plasma cells are a heterogenous cell population, comprised of several subsets: short-lived and proliferative plasma cell blasts in lymphoid tissues, transitional plasma cells in peripheral blood, long-lived and non-dividing plasma cells in bone marrow. Plasma cells of all differentiation stages are identified by their high expression level of the CD38 antigen. Blood plasma cells lack the expression of CD22, the typical B cell marker, and were found to express lower levels of CD19 than mature B cells. A subset of CD38+ blood plasma cells also expresses the CD138 antigen. |
| Applications |
| Isolated blood plasma cells can be used for investigations of plasma cell maturation and differentiation, their survival and chemotactic behaviour, for example, by molecular analysis of differential gene and protein expression, characterization of surface markers or the analysis of antibody production. |
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| Figure 1 |
| Plasma cells were isolated from PBMCs using the Plasma Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator for the depletion step and two MS Columns and a MiniMACS™ Separator for the positive selection step. Aliquots of the different cell fractions were stained with CD19-APC and CD38-FITC, respectively. |
| PBMCs before separation |
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| Pre-enriched CD38+ plasma cells |
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| Enriched CD38+ plasma cells |
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