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| IgM+ Memory B Cell Isolation Kit |
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| Overview |
| The IgM+ Memory B Cell Isolation Kit has been developed for the isolation of IgM+ memory B cells from human PBMCs. |
| Details |
Background information
IgM+ memory B cells, defined as quiescent antigen-experienced B cells, are generated in response to T cell–dependent and T cell–independent antigens. They are able to react quickly to a recurrent antigenic challenge thereby providing serological immune protection.
Detailed separation procedure
IgM+ memory B cells are isolated by depletion of unwanted cells and subsequent positive selection with Anti-IgM MicroBeads. Non-B cells (T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells) are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, and CD235a (Glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. |
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| Figure 1 |
| IgM+ memory B cells were isolated from human PBMCs using the IgM+ Memory B Cell Isolation Kit, an LD Column and a MidiMACS™ Separator, an MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-IgM-APC and CD27-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| Before separation |
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| Pre-enriched memory B cells |
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| Isolated IgM+ memory B cells |
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| Details |
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| Products |
| IgM+ Memory B Cell Isolation Kit, human |
- for 2×109 total cells
Download datasheet
130-093-619
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