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| Overview |
| The CD10 MicroBead Kit was developed for the enrichment or depletion of CD10+ cells from bone marrow or other cell sources. |
| Details |
Background information
CD10, the human common acute lymphoblastic leukemia antigen (CALLA), is a 100 kDa cell surface molecule identical to human membrane-associated neutral endopeptidase (NEP) and also known as neprilysin or enkephalinase. Human CD10 is expressed on a wide variety of normal and neoplastic cell types from different tissues including neural and hematopoietic cells.
CD10 is expressed on pre– and pro–B cells and is involved in B cell development and differentiation. The antigen is also present on mature neutrophils, T cell precursors, some T cell leukemias/lymphomas and on neoplastic cells of several B cell leukemias/lymphomas.¹,²
Applications
The CD10 MicroBead Kit can also be used for the isolation of CD10+ B cells from B cell leukemias/lymphomas. |
| Columns |
For positive selection: MS, LS, XS, or autoMACS Columns.
For depletion: LD, CS, D, or autoMACS Columns. |
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| CD10+ cells were isolated from human PBMCs spiked with Raji Burkitt´s lymphoma cells using the CD10 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Biotin-APC and CD19-PE and analyzed by flow cytometry. |
| Before separation |
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| After separation |
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| Details |
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| References |
| 1. Shipp and Look (1993) Blood 82: 1052–1070. |
| 2. Kalled et al. (1995) Eur. J. Immunol. 25: 677–687. |
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