| Description |
| Anti-IgG MicroBeads, formerly known as Mouse Anti-Human IgG MicroBeads, were developed for the isolation or depletion of surface IgG-expressing B cell populations from PBMCs or single-cell suspensions from lymphoid tissues. |
| Applications |
| Enrichment or depletion of memory B cells that express surface IgG can be achieved by sequential cell sorting using Anti-IgG MicroBeads. After the enrichment of B cells with the B Cell Isolation Kit II or the CD19 MultiSort Kit, IgG-expressing memory B cells are specifically labeled and isolated with Anti-IgG MicroBeads. A special protocol is available. |
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| Figure 1 |
| IgG+ memory B cells were separated from PBMCs. CD19+ B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator. |
| Pre-enriched CD19+ cells |
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| Isolated surface IgG+ memory B cells |
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