| Description |
| CD27 MicroBeads were developed for the magnetic separation of cells based on the expression of the CD27 antigen. CD27, a member of the tumor necrosis factor receptor family, is differentially expressed on B cells, T cells, and NK cells. Among peripheral blood B cells, CD27 is present on memory B cells and plasma cells but not on naive B cells. In human tonsils, expression of the CD27 antigen is found on memory B cells, germinal center B cells, subepithelial marginal zone B cells, and plasma cells. |
| Applications |
| CD27 MicroBeads are applied for positive selection or depletion of, for example, memory B cells from peripheral blood1,2 and lymphoid tissues3,4 by sequential magnetic cell separation. B lineage cells are first pre-selected by using the CD19 MultiSort Kit1 or the B Cell Isolation Kit II2. Subsequently, memory B cells are positively selected or depleted by using CD27 MicroBeads. CD27 MicroBeads can be further used for the isolation of specific T cell subsets or NK cell subsets. |
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| Figure 1 |
| Positive selection of CD19+CD27+memory B cells from human PBMCs. CD19+ B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population. |
| Pre-selected CD19+ cells |
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| CD19+CD27+ cells |
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