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| Overview |
| CD27 MicroBeads were developed for the positive selection or depletion of CD27+ cells from human PBMCs or lymphoid tissues. CD27 is differentially expressed on B cells, T cells, and NK cells. |
| Details |
Background information
CD27 is a member of the tumor necrosis factor receptor family, which among peripheral blood B cells is present on memory B cells1 and plasma cells but not on naive B cells. In human tonsils, expression of the CD27 antigen is found on memory B cells, germinal center B cells, subepithelial marginal zone B cells, and plasma cells. CD27 is further expressed on specific T cell subsets and NK cell subsets.
Applications
T cells can be pre-selected using the CD8+ T Cell Isolation Kit or the CD4+ T Cell Isolation Kit and subsequently separated according to CD27 expression.
For the isolation of human memory B cells, we recommend the Memory B Cell Isolation Kit. |
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| Figure 1 |
| Positive selection of CD19+CD27+memory B cells from human PBMCs. CD19+ B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population. |
| Pre-selected CD19+ cells |
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| CD19+CD27+ cells |
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| Details |
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| References |
| 1. De Milito et al. (2004) Blood 103: 2180-2186. |
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