| Overview |
| The Naive B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched naive B cells from human PBMCs. |
| Details |
Detailed separation procedure
Non-B cells (T cells, NK cells, dendritic cells, monocytes, granulocytes, platelets, and erythroid cells) and non-naive B cells (memory B cells, activated B cells, and plasma cells) are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD27, CD36, CD43, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Highly pure naive B cells are obtained by depletion of the magnetically labeled cells.
Downstream applications
Purified naive B cells can be used for studies in cell biology or molecular biology and functional assays. |
| |
| Figure 1 |
| Untouched naive B cells were isolated from human PBMCs using the Naive B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with a B cell marker, CD19-FITC, and anti-IgD-APC and CD27-PE to discriminate naive B cells (CD19+IgD+, A and C) from other B cells, i.e., memory B cells, activated B cells, and plasma cells (CD19+CD27+, B and D). |
| Before separation |
| A |
 |
| B |
 |
| Untouched naive B cells |
| C |
 |
| D |
 |
|
| Figure 2 |
| Isolation of untouched naive B cells using the Naive B Cell Isolation Kit II. |
 |
|
|
