| Description |
The Naive B Cell Isolation Kit II is an indirect magnetic labeling system for the isolation of untouched naive B cells from human PBMCs. Non-B cells, i.e., T cells, NK cells, dendritic cells, monocytes, granulocytes, platelets, and erythroid cells, and non-naive B cells, i.e., memory B cells, activated B cells, and plasma cells, are indirectly magnetically labeled using a cocktail of biotinylated CD2, CD14, CD16, CD27, CD36, CD43, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Highly pure naive B cells are obtained by depletion of magnetically labeled cells.
For evaluation of MACS® Separations, staining with fluorochrome-conjugated Anti-Biotin antibodies (Anti-Biotin-PE or -APC), CD19 antibodies, and anti-IgD antibodies is recommended. |
| Applications |
| Naive B cells isolated with the Naive B Cell Isolation Kit II are used in studies in which direct labeling of cell surface molecules could interfere with downstream applications. Purified naive B cells can be used for cell biological or molecular biological studies and functional assays. |
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| Figure 1 |
| Untouched naive B cells were isolated from human PBMCs using the Naive B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with a B cell marker, CD19-FITC, and anti-IgD-APC and CD27-PE to discriminate naive B cells (CD19+IgD+, A and C) from other B cells, i.e., memory B cells, activated B cells, and plasma cells (CD19+CD27+, B and D). |
| Before separation |
| A |
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| B |
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| Untouched naive B cells |
| C |
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| D |
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| Figure 2 |
| Isolation of untouched naive B cells using the Naive B Cell Isolation Kit II. |
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