| Overview |
| CD43 MicroBeads were developed for the isolation of untouched resting human B cells from PBMCs or lymphoid tissues, based on the magnetic depletion of cells that express the CD43 antigen. |
| Details |
Background information
CD43 (leukosialin, sialophorin) is a cell surface glycoprotein that is involved in adhesion, anti-adhesion, and signal transduction processes. The CD43 antigen is expressed on most leukocytes, i.e., T cells, NK cells, granulocytes, monocytes, and macrophages, on hematopoietic stem cells, and on platelets, but not on erythrocytes. Among B cells, CD43 is expressed on activated B cells and plasma cells but not on resting B cells, e.g., naive B cells. In bone marrow, CD43 is found on pro-B cells but is down-regulated during transition to the pre-B cell stage.
Downstream applications
Resting B cells, isolated with CD43 MicroBeads, can be used for studies on signal transduction processes, e.g., B cell activation or differentiation, and related molecular processes. The magnetically unlabeled CD43– cells may also be used for further sorting of B cell subsets using MACS® Technology. |
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| Figure 1 |
| Isolation of untouched CD43– B cells from PBMCs by depletion with CD43 MicroBeads using an LS Column and a MidiMACS™ Separator. |
| Before separation |
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| CD19+CD43– B cells |
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| CD19+CD43– B cells |
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