B Cell Isolation Kit II
Description The B Cell Isolation Kit II is an indirect magnetic labeling system for the isolation of untouched B cells from human PBMCs. Non-B cells, i.e., T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells, are indirectly magnetically labeled using a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Highly pure B cells are obtained by depletion of magnetically labeled non-B cells. For evaluation of MACS® Separations, staining with fluorochrome-conjugated Anti-Biotin antibodies (Anti-Biotin-PE or -APC) and CD19 antibodies is recommended. Applications B cells isolated with the B Cell Isolation Kit II are used in studies in which direct labeling of B cell surface molecules could interfere with downstream applications. Untouched B cells may also be used for the isolation of specific B cell subsets, including naive and memory B cells.1,2,3 Columns LS or autoMACS™ Columns.		Figure 1 Untouched B cells were isolated from PBMCs using the B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Before separation Untouched B cells Figure 2 Scanning electron micrograph of a B cell isolated with the B Cell Isolation Kit. (Courtesy of Prof. Groscurth, Zürich, Switzerland). Figure 3 Isolation of untouched B cells using the B Cell Isolation Kit II.