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 - Reliable: excellent reproducibility avoids cumbersome and expensive experiment repetitions
- Proven: successfully applied in basic and translational research
- Straightforward: optimal for subsequent B cell subset isolation
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| Overview |
| The B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched B cells from human PBMCs. |
| Details |
Detailed separation procedure
Non-B cells (T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells) are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Highly pure B cells are obtained by depletion of magnetically labeled non-B cells.
Downstream applications
Untouched B cells obtained with the B Cell Isolation Kit II can be used for the isolation of specific B cell subsets, including naive and memory B cells.1,2,3 |
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| Figure 1 |
| Untouched B cells were isolated from PBMCs using the B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. |
| Before separation |
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| Untouched isolated B cells |
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| Figure 2 |
Scanning electron micrograph of a B cell isolated with the B Cell Isolation Kit.
(Courtesy of Prof. Groscurth, Zürich, Switzerland). |
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| Figure 3 |
| Isolation of untouched B cells using the B Cell Isolation Kit II. |
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| Favorites / Prices |
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| Details |
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| Products |
| B Cell Isolation Kit II, human |
- for 109 total cells
Download datasheet
130-091-151
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| References |
| 1. Poeck et al. (2004) Blood 103: 3058-3064. |
| 2. Marinkovic (2004) Nucleics Acids Research 32: 5368–5378. |
| 3. Jaimes (2004) J. Virol. 78: 10967–10976. |
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