CD22 MicroBeads
Description CD22 MicroBeads were developed for magnetic isolation of human B cells. In peripheral blood, cell surface expression of the CD22 antigen is found on mature B cells, but it is lost during terminal differentiation to plasma cells. In bone marrow, surface expression of CD22 can be detected from the pre-B cell stage on. Cytoplasmic expression of CD22 can also be found in late pro-B cells and early pre-B cells. Applications CD22 MicroBeads can be used for positive selection or depletion of human B cells from PBMCs2, bone marrow3, or single-cell suspensions from lymphoid tissues1. The purified B cells may be used for cell biological studies, e.g., on signal transduction2, mixed lymphocyte responses1, antigen presentation, or for molecular studies, such as PCR analysis3. Columns For positive selection: MS, LS, XS, or autoMACS™ Columns; for depletion: LD, CS, D, or autoMACS Columns.		Figure 1 Separation of CD22+ cells from PBMCs using CD22 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are stained with CD19-FITC. Figure 2 B cells isolated from PBMCs using MACS® Technology, Giemsa stained.

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