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| Overview |
| CD22 MicroBeads were developed for positive selection or depletion of human B cells from PBMCs, bone marrow, or single-cell suspensions from lymphoid tissues. |
| Details |
Background information
In peripheral blood, cell surface expression of the CD22 antigen is found on mature B cells, but is lost during terminal differentiation to plasma cells. In bone marrow, surface expression of CD22 can be detected from the pre-B cell stage on. Cytoplasmic expression of CD22 can also be found in late pro-B cells and early pre-B cells.
Downstream applications
The purified B cells may be used for cell biological studies, such as analysis of signal transduction2, mixed lymphocyte responses1, antigen presentation, or for molecular studies, e.g. PCR analysis3. |
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| Figure 1 |
| Separation of CD22+ cells from PBMCs using CD22 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are stained with CD19-FITC. |
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| Figure 2 |
| B cells isolated from PBMCs using MACS® Technology, Giemsa stained. |
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| Details |
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| References |
| 1. Butch et al. (1999) Blood 94: 216-224. |
| 2. Jacquot et al. (2001) Int. Immunol. 13: 871-876. |
| 3. Borson et al. (2002) Blood 100: 4629-4639. |
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