| Description |
| CD20 MicroBeads have been developed for the isolation of highly pure human B cells based on the expression of the CD20 antigen. CD20 is a non-glycosylated transmembrane protein of 33–37 kDa that becomes heavily phosphorylated upon activation1,2. It is expressed exclusively on B cells, including pre-B cells, naive, mature, and activated B cells, B cell blasts, and the majority of malignant B cells, but not on early B cell progenitors or plasma cells2,3. Unlike other anti-CD20 antibodies, the specific antibody clone coupled to MACS® MicroBeads induces neither apoptosis nor complement lysis.4 |
| Applications |
| CD20 MicroBeads can be used for positive selection or depletion of human B cells from many different cell sources such as peripheral blood, bone marrow, cell cultures, or single-cell suspensions from lymphoid tissues. Purified B cells may be used in cell biological studies, such as analysis of signal transduction, chemotaxis, allergen response, apoptosis, or in molecular biological studies, e.g., subset-specific microarrays. |
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| Figure 1 |
| B cells were separated from PBMCs using CD20 MicroBeads and the autoMACS™ Separator. |
| PBMCs before separation |
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| Enriched CD20+ cells |
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