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| Overview |
| The CD19 MultiSort Kit was developed for the isolation of CD19+ B cell subsets by sequential sorting from human peripheral blood, bone marrow, cell cultures, or single-cell suspensions from tissue. |
| Details |
Detailed separation procedure
Lineage B cells are isolated with CD19 MultiSort MicroBeads and subsequently the magnetic particles are enzymatically released. The selected CD19+ B cells can then be magnetically labeled and sorted according to a second antigen. The kit contains CD19 MultiSort MicroBeads, the MultiSort Release Reagent, and the MultiSort Stop Reagent.
Applications
The CD19 MultiSort Kit can be used for positive selection or depletion of B cell populations that express distinct developmental markers such as CD5 (e.g. B-1 cells), CD27 (e.g. memory B cells)1, or IgD (e.g. naive B cells)2. It can be further used for the isolation of activated B cells according to activation markers, e.g., CD25, CD30, CD38, or CD69. For selection of specific B cell subsets please refer also to the subset kits available. |
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| Figure 1 |
| Tetanus-toxin-specific CD19+ B cells were enriched from pre-selected peripheral blood CD19+ B cells. Cells were stained with CD19-FITC and Tetanus-toxin-PE. |
| PBMCs before separation |
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| Enriched CD19+ B cells |
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| Enriched Tetanus-toxin-specific CD19+ B cells |
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| Figure 2 |
| Step-by-step CD19 MultiSort separation procedure. |
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| Details |
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| References |
| 1. De Milito et al. (2004) Blood 103: 2180-2186. |
| 2. Al-Darmaki et al. (2004) Clin. Diagn. Lab. Immunol. 11: 720–728. |
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