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CD19 MultiSort Kit
Description
The CD19 MultiSort Kit is a direct magnetic labeling system for the isolation of distinct CD19+ B cell subsets from human PBMCs, bone marrow, or single-cell suspensions from lymphoid tissues. B lineage cells are isolated with CD19 MultiSort MicroBeads and subsequently the magnetic particles are enzymatically released. The selected CD19+ B cells can then be magnetically labeled and sorted according to a second antigen. The CD19 MultiSort Kit is a complete system for sequential positive selections. It contains CD19 MultiSort MicroBeads, the MultiSort Release Reagent, and the MultiSort Stop Reagent.
Applications
The CD19 MultiSort Kit can be used for positive selection or depletion of B cell populations that express distinct developmental markers such as CD5 (e.g. B-1 cells), CD27 (e.g. memory B cells)1, or IgD (e.g. naive B cells)2. It can be further used for the isolation of activated B cells according to activation markers, e.g., CD25, CD30, CD38, or CD69, or for the selection of B cell subsets according to the surface expression of Ig subclasses, e.g., IgG or IgM. Furthermore, the CD19 MultiSort Kit has been used to enrich antigen-specific B cells (fig.1).3,4,5 A special protocol is available.
Columns
For positive selection: MS, LS, XS, or autoMACS™ Columns.
Further information
Isolation of human surface IgG expressing memory B cells
[PDF; 117,9 KB]
 
Figure 1
Tetanus-toxin-specific CD19+ B cells were enriched from pre-selected peripheral blood CD19+ B cells. Cells were stained with CD19-FITC and Tetanus-toxin-PE.
PBMCs before separation
Enriched CD19+ B cells
Enriched tetanus-toxin-specific CD19+ B cells
Figure 2
Step-by-step CD19 MultiSort separation procedure.
A
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Products
CD19 MultiSort Kit, human
for 109 total cells
Download data sheet
130-055-301
Qty:
 

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Anti-IgG MicroBeads, human (#130-047-501)
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CD27 MicroBeads, human (#130-051-601)
CD30 MicroBeads, human (#130-051-401)
CD69 MicroBead Kit, human (#130-051-501)
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MACS References
1. De Milito et al. (2004) Blood 103: 2180-2186.[4112]
2. Al-Darmaki et al. (2004) Clin. Diagn. Lab. Immunol. 11: 720–728.[4304]
3. Leyendeckers et al. (2003) J. Invest Dermatol. 120: 372-378.[3991]
4. Leyendeckers et al. (2002) Eur. J. Immunol. 32: 3126-3132.[2773]
5. Leyendeckers et al. (1999) Eur. J. Immunol. 29: 1406-1417.[566]
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