| Description |
| The CD19 MultiSort Kit is a direct magnetic labeling system for the isolation of distinct CD19+ B cell subsets from human PBMCs, bone marrow, or single-cell suspensions from lymphoid tissues. B lineage cells are isolated with CD19 MultiSort MicroBeads and subsequently the magnetic particles are enzymatically released. The selected CD19+ B cells can then be magnetically labeled and sorted according to a second antigen. The CD19 MultiSort Kit is a complete system for sequential positive selections. It contains CD19 MultiSort MicroBeads, the MultiSort Release Reagent, and the MultiSort Stop Reagent. |
| Applications |
| The CD19 MultiSort Kit can be used for positive selection or depletion of B cell populations that express distinct developmental markers such as CD5 (e.g. B-1 cells), CD27 (e.g. memory B cells)1, or IgD (e.g. naive B cells)2. It can be further used for the isolation of activated B cells according to activation markers, e.g., CD25, CD30, CD38, or CD69, or for the selection of B cell subsets according to the surface expression of Ig subclasses, e.g., IgG or IgM. Furthermore, the CD19 MultiSort Kit has been used to enrich antigen-specific B cells (fig.1).3,4,5 A special protocol is available. |
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| Figure 1 |
| Tetanus-toxin-specific CD19+ B cells were enriched from pre-selected peripheral blood CD19+ B cells. Cells were stained with CD19-FITC and Tetanus-toxin-PE. |
| PBMCs before separation |
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| Enriched CD19+ B cells |
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| Enriched tetanus-toxin-specific CD19+ B cells |
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| Figure 2 |
| Step-by-step CD19 MultiSort separation procedure. |
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