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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
  NK cells
  B Cells
 
 
 
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD19 MultiSort Kit
Overview
The CD19 MultiSort Kit was developed for the isolation of CD19+ B cell subsets by sequential sorting from human peripheral blood, bone marrow, cell cultures, or single-cell suspensions from tissue.
Details
Detailed separation procedure
Lineage B cells are isolated with CD19 MultiSort MicroBeads and subsequently the magnetic particles are enzymatically released. The selected CD19+ B cells can then be magnetically labeled and sorted according to a second antigen. The kit contains CD19 MultiSort MicroBeads, the MultiSort Release Reagent, and the MultiSort Stop Reagent.

Applications
The CD19 MultiSort Kit can be used for positive selection or depletion of B cell populations that express distinct developmental markers such as CD5 (e.g. B-1 cells), CD27 (e.g. memory B cells)1, or IgD (e.g. naive B cells)2. It can be further used for the isolation of activated B cells according to activation markers, e.g., CD25, CD30, CD38, or CD69. For selection of specific B cell subsets please refer also to the subset kits available.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns.
 
Figure 1
Tetanus-toxin-specific CD19+ B cells were enriched from pre-selected peripheral blood CD19+ B cells. Cells were stained with CD19-FITC and Tetanus-toxin-PE.
PBMCs before separation
Enriched CD19+ B cells
Enriched Tetanus-toxin-specific CD19+ B cells
Figure 2
Step-by-step CD19 MultiSort separation procedure.
A
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Details
Products
CD19 MultiSort Kit, human
- for 109 total cells
Download datasheet
130-055-301
Qty.:
 

Related products
Anti-IgG MicroBeads, human (#130-047-501)
CD25 MicroBeads II, human (#130-092-983)
CD27 MicroBeads, human (#130-051-601)
CD30 MicroBeads, human (#130-051-401)
CD69 MicroBead Kit II, human (#130-092-355)
CD19 Antibodies
References
1. De Milito et al. (2004) Blood 103: 2180-2186.
2. Al-Darmaki et al. (2004) Clin. Diagn. Lab. Immunol. 11: 720–728.
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