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| Whole Blood CD19 MicroBeads |
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| Overview |
| Whole Blood CD19 MicroBeads allow the fast isolation of CD19+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis. |
| Details |
Background information
The CD19 antigen is expressed from the early pro-B cell to the B cell blast stage. It is also present on most malignant B cells. CD19 expression is down-regulated upon terminal differentiation of B cells to plasma cells.
Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD19 MicroBeads and subsequently separated with the autoMACS® Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit.
Using the Whole Blood MicroBeads in combination with the autoMACS® Pro Separator or the autoMACS Separator allows standardization of the cell separation procedure and safe handling of hazardous samples.
Downstream applications
The purified CD19+ cells are well suited for flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2,3. |
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| Figure 1 |
| CD19+ cells were separated from whole blood using Whole Blood CD19 MicroBeads and the autoMACS® Separator with program Posseld2. Cells were fluorescently stained with CD20-FITC and CD45-PE. |
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| Favorites / Prices |
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| Details |
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| Products |
| Whole Blood CD19 MicroBeads, human |
| For research use only |
- for 40 mL whole blood
Download datasheet
130-090-880
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| References |
| 1. Köhl et al. (2003) Leukemia 17: 232–236. |
| 2. Adams et al. (2004) MACS&more 8/2: 16–17. |
| 3. Meyer et al. (2006) Blood 109: 374–382. |
| 4. Willasch et al. (2010) Bone Marrow Transplantation 45: 181–189. |
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