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| B Cell Isolation Kit (B-CLL) |
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| Overview |
| The B Cell Isolation Kit (B-CLL) was developed for the fast and efficient isolation of untouched B cells from PBMCs. The kit has been especially designed for the isolation of B cells from PBMCs of B-CLL or other B cell malignancies. |
| Details |
Detailed separation procedure
Non-B cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD2, CD4, CD11b, CD16, CD36, Anti-IgE, and CD235a (Glycophorin A), and Anti-Biotin MicroBeads. Isolation of untouched B cells is achieved by depletion of magnetically labeled cells. The depletion cocktail does not contain CD43, which might be expressed on the target cells.
Downstream applications
The B Cell Isolation Kit (B-CLL) can be used for studies on B cells from tumor cell-containing samples and for functional studies on B cells. Furthermore the kit can be used for studies on signal requirements for B cell activation, induction of B cell proliferation, differentiation of B cells, induction of apoptosis in B cells, etc. Further applications include studies on signal transduction in B cells and the analysis of immunoglobulin class switching and somatic hypermutation in B cells. |
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| Figure 1 |
| Isolation of untouched B cells from cryopreserved human PBMCs containing B-CLL cells using the B Cell Isolation Kit (B-CLL), an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC and Anti-Biotin-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The data were provided by Dr. E.Distler and Dr. R.G. Meyer, Johannes Gutenberg-Universität Mainz, Germany. |
| Before separation |
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| Untouched isolated B cells |
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| Favorites / Prices |
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| Details |
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| Products |
| B Cell Isolation Kit (B-CLL), human |
- for 109 total cells
Download datasheet
130-093-660
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