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| Overview |
| CD45 MicroBeads were developed for the positive selection or depletion of leukocytes from peripheral blood, erythrocyte and thrombocyte preparations, lymphoid tissues, tumor tissues, as well as nonhematopoietic tissues. |
| Details |
Background information
The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes, platelets, and their precursor cells.
Applications
CD45 MicroBeads have been used to enrich fetal erythroblasts from maternal blood. They were also used to enrich epithelial tumor cells from peripheral blood, bone marrow, or lymphoid tissue by depletion of CD45+ leukocytes1. Pluripotent stem cells from human bone marrow have been enriched by depletion of cells labeled with CD235a (Glycophorin A) MicroBeads and CD45 MicroBeads.2 Furthermore, CD45 MicroBeads have been used in xenogeneic engraftment studies for the isolation of CD45+ leukocytes. |
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| Figure 1 |
| Separation of human leukocyte-enriched buffy coat using CD45 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are stained with CD45-FITC. |
| Peripheral blood before separation |
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| CD45– cells |
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| CD45+ cells |
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| Figure 2 |
| A cytokeratin-positive cell was enriched from bone marrow of a patient with renal carcinoma by depletion of leukocytes using CD45 MicroBeads. Cells were stained using a conventional APAAP method followed by staining with hematoxylin. (Courtesy of Dr. Bilkenroth, Dept. Pathol. and Dr. Meye, Dept. Urol., Univ. Halle, Germany.) |
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| Favorites / Prices |
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| Details |
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| Products |
| CD45 MicroBeads, human |
| For research use only |
- for 109 total cells
Download datasheet
130-045-801
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| References |
| 1. Meye et al. (2002) Int. J. Oncol. 21: 521-530. |
| 2. Verfaillie et al. (2002) Nature 418: 41-49. |
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