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| Whole Blood CD45 MicroBeads |
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| Overview |
| Whole Blood CD45 MicroBeads were developed for rapid positive selection of CD45+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis. |
| Details |
Background information
The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes, platelets, and their precursor cells.
Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD45 MicroBeads and subsequently separated with the autoMACS® Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit. Whole Blood MicroBeads in combination with the autoMACS® Separators allow standardization of the cell separation procedure and safe handling of hazardous samples.
Downstream applications
The purified CD45+ cells are well suited for flow cytometric, functional, or molecular analysis including chimerism analysis after allogeneic stem cell transplantation1. |
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| Figure 1 |
| Leukocytes were separated from whole blood using Whole Blood CD45 MicroBeads and the autoMACS® Separator with program Posseld2. Cells were fluorescently stained with CD45-FITC and CD235a (Glycophorin A)-PE. |
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| Favorites / Prices |
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| Details |
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| Products |
| Whole Blood CD45 MicroBeads, human |
| For research use only |
- for 40 mL whole blood
Download datasheet
130-090-872
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| References |
| 1. Köhl et al. (2003) Leukemia 17: 232–236. |
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