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| Indirect CD133 MicroBead Kit |
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| Description |
The Indirect CD133 MicroBead Kit contains all reagents necessary for the isolation of CD133+ human cells. The kit is based on the use of an indirect labeling system; cells are first labeled with a biotinylated CD133/1 primary antibody (clone AC133) and then with Anti-Biotin MicroBeads in a second step. The kit also contains FcR Blocking Reagent to prevent nonspecific magnetic labeling of cells via Fc receptors.
The CD133 molecule is a 5-transmembrane cell surface antigen with a molecular weight of 117 kD.1 The CD133/1 (clone AC133) antibody recognizes epitope 1 of the CD133 antigen.2,3 In the hematopoietic system, CD133 expression is restricted to a subset of CD34bright stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood.4 Additionally, CD133 is expressed by a small portion of CD34– cells in these tissues.5 The CD34+CD133+ cell population, which includes CD34+CD38– cells, was shown to be capable of repopulating NOD/SCID mice.6 Recently, CD133 has also been found to be expressed on circulating endothelial progenitor cells7,8 and fetal neural stem cells9,10 as well as on other tissue-specific stem cells, such as renal11, prostate12, and corneal13 stem cells. The putative murine homologue, prominin, is expressed on neuroepithelial and epithelial mouse cells.14 |
| Applications |
| Positive selection of CD133+ cells from peripheral blood mononuclear cells (PBMCs), bone marrow aspirate, cord blood, and single-cell suspensions of nonhematopoietic tissues. |
| Columns |
| MS, LS, XS, or autoMACS™ Columns |
| Clone | Isotype |
| AC133 | Mouse IgG1 |
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| Figure 1 |
| Separation of non-mobilized PBMCs using CD133/1 (AC133)-Biotin, Anti-Biotin MicroBeads, MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with CD34-FITC and CD133/2 (293C3)-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
| Before separation |
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CD133+ cells
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| Products |
| Indirect CD133 MicroBead Kit, human |
for 2Ă—109 total cells
for 20 tests
Download data sheet
130-091-895
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| MACS References |
| 1. Miraglia, S. et al. (1997) Blood 90: 5013–5021.[382] |
| 2. Yin, A. H. et al. (1997) Blood 90: 5002–5012.[383] |
| 3. Piechaczek, C. (2001) J Biol Reg Hom Agents 15: 101–102.[1039] |
| 4. Bühring, H. J. et al. (1999) Ann NY Acad Sci: 872: 25–39.[1015] |
| 5. Gallacher, L. et al. (2000) Blood 95: 2813–2820.[698] |
| 6. de Wynter, E. A. et al. (1998) Stem Cells 16: 387–396.[503] |
| 7. Gehling, U. M. et al. (2000) Blood 95: 3106–3112.[934] |
| 8. Peichev, M. et al. (2000) Blood 95: 952–958.[931] |
| 9. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97: 14720–14725.[933] |
| 10. Cummings, B. J. et al. (2005) Proc. Natl. Acad. Sci. USA 102: 14069–14074. |
| 11. Bussolati, B. et al. (2005) Am. J. Pathol. 166: 545–555. |
| 12. Richardson, G. et al. (2004) J. Cell Sci. 117: 3539–3545.[7531] |
| 13. Thill, M. et al. (2004) Invest. Opthalmol. Vis. Sci. 45: 3519.[7392] |
| 14. Weigmann, A. et al. (1998) Proc. Natl. Acad. Sci. USA 94: 12425–12430.[442] |
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