| Description |
The CD34 MultiSort Kit is a complete system for the sequential positive selection of subpopulations of CD34+ hematopoietic stem cells. The kit contains CD34 MultiSort MicroBeads, FcR Blocking Reagent to avoid unspecific labeling, the MultiSort Release Agent, and MultiSort Stop Reagent.
In the first dimension, CD34+ cells are positively selected from samples such as peripheral blood, bone marrow aspirate, leukapheresis product, or cord blood. However, with the CD34 MultiSort Kit, magnetically labeled cells can be sorted for a second time using a different selection marker after the enzymatic release of the CD34 MultiSort MicroBeads from the cell surface.
Fluorescent control staining of magnetically labeled cells requires a monoclonal CD34 antibody recognizing an epitope other than QBEND/10, for example clone AC136 (see CD34 antibodies). |
| Applications |
| The CD34 MultiSort Kit can be used to isolate specific subsets of CD34+ cells. The CD34-selected cells can be further sorted according to their expression of, for example, HLA-DR, CD4, CD10, CD38, CD45RA, CD61, CD71, CD90 (Thy1), CD117, or CD133. |
| Clone | Isotype |
| QBEND/10 | Mouse IgG1 |
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| Figure 1 |
| CD34+ HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34+ cells from PBMCs was followed by the depletion of CD34+HLA-DR+ cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34+HLA-DR– cells (right panel, red). |
| A: PBMCs before separation |
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| B: CD34+ cells |
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| C: CD34+HLA-DR- cells |
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| Figure 2 |
| Step-by-step CD34 MultiSort separation procedure. |
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| Figure 3 |
| CD34+ lin- cells isolated from peripheral blood using CD34 MultiSort Kit, May-GrĂĽnwald-Giemsa stained. (Courtesy of Dr. Ziegler, TĂĽbingen, Germany.) |
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