| Overview |
The CD34 MultiSort Kit has been developed for the positive selection of CD34+ subpopulations by sequential sorting. CD34+ hematopoietic stem and progenitor cells can be isolated from
- peripheral blood,
- bone marrow aspirate,
- leukapheresis product,
- cord blood,
- differentiated ES and iPS cell cultures1.
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| Details |
Detailed separation procedure
Using the CD34 MultiSort Kit, in the first step CD34+ cells are positively selected after labeling with the CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34+ cells can be magnetically labeled and sorted according to a second antigen. The kit contains CD34 MultiSort MicroBeads, MultiSort Release Reagent, and MultiSort Stop Reagent.
Control staining after separation requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136
Downstream applications
The CD34 MultiSort Kit is used to isolate specific subsets of CD34+ hematopoietic stem and progenitor cells. CD34-selected cells can be further sorted according to their expression of, e.g., HLA-DR, CD43, CD45RA, CD61, CD71, CD90 (Thy1), or CD133. |
| Clone | Isotype |
| QBEND/10 | Mouse IgG1 |
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| Figure 1 |
| CD34+ HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34+ cells from PBMCs was followed by the depletion of CD34+HLA-DR+ cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34+HLA-DR– cells (right panel, red). |
| A: PBMCs before separation |
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| B: CD34+ cells |
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| C: CD34+HLA-DR- cells |
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| Figure 2 |
| Step-by-step CD34 MultiSort separation procedure. |
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| Figure 3 |
| CD34+ lin- cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.) |
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