| Description |
| The Lineage Cell Depletion Kit is an indirect magnetic labeling system for the depletion of mature hematopoietic cells such as T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, erythroid cells, and their committed precursors from bone marrow aspirate, cord blood, or mobilized leukapheresis product. The depletion of lineage-committed cells results in the enrichment of untouched stem and progenitor cells. |
| Details |
Detailed separation procedure
Cells are first labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens (CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a (Glycophorin A)). These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells.
Downstream applications
- Flow cytometric characterization of rare stem and progenitor cell subsets
- Evaluation of hematopoietic differentiation potential in vitro using CFU assays (CFC, CFU-GM, BFU-E, and CFU-Mk)
- Evaluation of the CFU-F potential
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| Figure 1 |
| Isolation of untouched lineage-negative cells from bone marrow mononuclear cells (BM-MNCs) using the Lineage Cell Depletion Kit and an LS Column. Cells were fluorescently stained with CD34-FITC. |
| BM-MNCs before separation |
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| Lineage+ fraction |
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| CD34+ cells in the lineage cell–depleted fraction. |
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