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 - High impact: tool of choice in more than 2,000 publications
- Fast: cell isolation in less than one hour
- Flexible: Isolate target cells from peripheral blood, bone marrow, cord blood, differentiated ES or iPS cells and many more tissues
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| Overview |
The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:
- peripheral blood (PBMCs)
- bone marrow1
- leukapheresis product2
- cord blood3
- differentiated ES and iPS cell lines4,5.
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| Details |
Background information
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts5,6, endothelial progenitor cells5,7-9, and mature endothelial cells8.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.
Downstream applications
Isolated cells are suitable for various downstream applications, e.g., flow cytometric analysis, expansion, transduction10 or differentiation5 in culture, reprogramming3, gene expression analyses, or transplantation into humanized mice. |
| Clone | Isotype |
| QBEND/10 | Mouse IgG1 |
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| Figure 1 |
Flow cytometric analysis of CD34+ cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.
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| A: BM-MNCs before separation |
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| B: Isolated CD34+ cells |
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| Favorites / Prices |
Request prices or add products to your favorite list.
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| Details |
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| Products |
| CD34 MicroBead Kit, human |
- for 2×109 total cells
Components
- 2 mL CD34 MicroBeads, human
- 2 mL FcR Blocking Reagent
Download datasheet
130-046-702
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- for 1010 total cells
Components
- 10 mL CD34 MicroBeads, human
- 10 mL FcR Blocking Reagent
Download datasheet
130-046-703
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| CD34 MicroBead Kit, human – lyophilized |
- for 2x109 total cells
Components
- 1 vial CD34 MicroBeads, human - lyophilized
- 2 mL Resuspension Buffer
- 2 mL FcR Blocking Reagent,
Download datasheet
130-097-047
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| References |
| 1. Schreiber et al. (2009) Hematologica 94: 1493-1501 |
| 2. Jing et al. (2010) Bone Marrow Transplant 45: 1489-1496. |
| 3. Ban et al. (2011) Proc Natl Acad Sci USA 108: 14234-9 |
| 4. Bandi and Akkina (2008) AIDS Res. Ther. 5: 1. |
| 5. Wang et al. (2007) Nat. Biotechnol. 25: 317–318. |
| 6. Loges et al. (2004) Stem Cells Dev. 13: 229–242. |
| 7. Peichev et al. (2000) Blood 95: 952–958. |
| 8. Rafii and Lyden (2003) Nat. Med. 9: 702–712. |
| 9. Valgimigli et al. (2004) Circulation 110:1209–1212. |
| 10. Chen et al. (2011) Blood 117: 1530-1539. |
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