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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
 
 
 
 
 
 
  T cells
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD34 MicroBead Kit

CD34 MicroBead Kit

  • High impact: tool of choice in more than 2,000 publications
  • Fast: cell isolation in less than one hour
  • Flexible: Isolate target cells from peripheral blood, bone marrow, cord blood, differentiated ES or iPS cells and many more tissues
Overview
The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:

  • peripheral blood (PBMCs)
  • bone marrow1
  • leukapheresis product2
  • cord blood3
  • differentiated ES and iPS cell lines4,5.
Details
Background information
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts5,6, endothelial progenitor cells5,7-9, and mature endothelial cells8.

Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.

Downstream applications
Isolated cells are suitable for various downstream applications, e.g., flow cytometric analysis, expansion, transduction10 or differentiation5 in culture, reprogramming3, gene expression analyses, or transplantation into humanized mice.
Columns
MS, LS, XS, or autoMACS Columns.
Clone Isotype
QBEND/10Mouse IgG1
 
Figure 1
Flow cytometric analysis of CD34+ cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.
A: BM-MNCs before separation
B: Isolated CD34+ cells
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Details
Products
CD34 MicroBead Kit, human
- for 2×109 total cells
Components
- 2 mL CD34 MicroBeads, human
- 2 mL FcR Blocking Reagent
Download datasheet
130-046-702
Qty.:
 

- for 1010 total cells
Components
- 10 mL CD34 MicroBeads, human
- 10 mL FcR Blocking Reagent
Download datasheet
130-046-703
Qty.:
 

CD34 MicroBead Kit, human – lyophilized
- for 2x109 total cells
Components
- 1 vial CD34 MicroBeads, human - lyophilized
- 2 mL Resuspension Buffer
- 2 mL FcR Blocking Reagent,
Download datasheet
130-097-047
Qty.:
 

Related products
CD34 Antibodies
MC CD34 Stem Cell Cocktail, human (#130-093-427)
MC CD34/CD133 Stem Cell Cocktail, human (#130-092-882)
Diamond CD34 Isolation Kit, human (#130-094-531)
CD34 MultiSort Kit, human (#130-056-701)
MACS® HSC-CFU Media
CD133/2 Antibodies
Antibodies for analysis of human HSCs
References
1. Schreiber et al. (2009) Hematologica 94: 1493-1501
2. Jing et al. (2010) Bone Marrow Transplant 45: 1489-1496.
3. Ban et al. (2011) Proc Natl Acad Sci USA 108: 14234-9
4. Bandi and Akkina (2008) AIDS Res. Ther. 5: 1.
5. Wang et al. (2007) Nat. Biotechnol. 25: 317–318.
6. Loges et al. (2004) Stem Cells Dev. 13: 229–242.
7. Peichev et al. (2000) Blood 95: 952–958.
8. Rafii and Lyden (2003) Nat. Med. 9: 702–712.
9. Valgimigli et al. (2004) Circulation 110:1209–1212.
10. Chen et al. (2011) Blood 117: 1530-1539.
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