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| Diamond CD133 Isolation Kit |
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| Overview |
| The Diamond CD133 Isolation Kit was specifically developed for the isolation of highly pure Lin–CD133+ stem cells from peripheral blood, cord blood, bone marrow, and leukapheresis product by sequential sorting. |
| Details |
Detailed separation procedure
The Diamond CD133 MicroBead Kit includes a lineage depletion followed by a CD133 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD133+ cells are isolated from the lineage-negative stem and progenitor cells using CD133 MicroBeads. The resulting population obtained are Lin-CD133+ stem cells.
Diamond CD133 MicroBeads recognize the epitope CD133/1. For control staining of separated cells, the application of CD133/2 (293C3) antibody is recommended.
Downstream applications
Lin-CD133+stem cells can be used for many applications, including:
- gene or miRNA expression profiling
- characterization of undifferentiated stem cells and their developmental potential
- stimulation of proliferation and maturation by cytokines
- HSC-CFU assays
- adoptive transfer and hematopoietic reconstitution studies
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| Favorites / Prices |
Request prices or add products to your favorite list.
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| Details |
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| Products |
| Diamond CD133 Isolation Kit, human |
- for 2×109 total cells
Components
- 2 mL Diamond Lin Biotin-Antibody Cocktail, human
- 2 x 2 mL Anti-Biotin MicroBeads
- 2 mL CD133 Diamond MicroBeads, human
Download datasheet
130-094-913
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