Diamond CD133 Isolation Kit
Description The Diamond CD133 Isolation Kit was specifically designed for the isolation of highly pure CD133+Lin– stem cells. CD133 is a marker found on undifferentiated adult stem cells, which have the capacity to differentiate into various cell lineages. CD133+ stem cells can be isolated from peripheral blood, cord blood, bone marrow, and leukapheresis product. The Diamond CD133 Isolation Kit, human, combines a lineage depletion and a CD133+ cell enrichment to obtain pure CD133+Lin– stem cells. CD133 MicroBeads recognize the epitope CD133/1. Using the Diamond CD133 Isolation Kit, human, primitive CD133 cells can be isolated from BM-MNCs or PBMCs even if the starting frequency is low. For quality control staining of separated cells using the Diamond CD133 Isolation Kit, the application of CD133/2 (293C3)-PE or -APC is recommended. Visit our stem cell website to find out more about our range of solutions for stem cell research. Applications The highly purified CD133+Lin- stem cells can be used for many applications, including: gene or miRNA expression profiling characterization of undifferentiated stem cells and their developmental potential stimulation of proliferation and maturation by cytokines HSC-CFU assay potential adoptive transfer and hematopoietic reconstitution studies Columns For positive selection: MS, or autoMACS® Columns; for depletion: LD, or autoMACS Columns.		Figure 1 CD133+Lin– cells were isolated from human PBMCs using the Diamond CD133 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD133/2 (293C3)-PE, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. PBMCs before separation Lineage-negative fraction Isolated CD133+Lin- cells

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