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| MSC Research Tool Box - MSCA-1 (W8B2) |
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| Overview |
| The MSC Research Tool Box – MSCA-1 (W8B2), based on the antibody clone W8B2, was developed for the standardized and optimized enrichment and expansion of MSCs with a high proliferative capacity from bone marrow aspirates. |
| Details |
Background information
The Anti-MSCA-1 (W8B2) MicroBeads recognize the mesenchymal stromal cell antigen-1 (MSCA-1), which is identical to tissue non-specific alkaline phosphatase1.
MSCA-1 was shown to be restricted to MSCs in the CD271bright population in bone marrow2. These CD271brightCD45dim MSCs have a much higher clonogenic capacity compared to the CD271+CD45+ fraction in bone marrow. Therefore, MSCA-1 is a highly specific marker for the isolation of MSCs with a high proliferative potential from bone marrow. MSCA-1 expression was not found on placenta-derived MSCs after culture3.
Antibodies against MSCA-1 show broad interspecies cross-reactivity such as dog, pig, sheep, goat and monkey4.
Detailed procedure
The tool box contains all necessary reagents for the magnetic isolation of MSCA-1+ cells, including Anti-MSCA-1 (W8B2) MicroBeads for magnetic labeling and FcR Blocking Reagent to block non-specific labeling via Fc receptors, NH Expansion Medium, a specialized medium for the expansion of MSCs, and CytoMix – MSC, a cytokine cocktail designed specifically for the optimal and reproducible expansion of MSCs. |
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| Isolation of MSCs from bone marrow mononuclear cells (BM-MNCs) using Anti-MSCA-1 (W8B2) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC. |
| Before separation |
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| MSCA-1– cells |
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| MSCA-1+ cells |
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| Favorites / Prices |
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| Details |
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| Products |
| MSC Research Tool Box - MSCA-1 (W8B2), human |
- for 109 total cells
Download datasheet
130-093-572
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| References |
| 1. Sobiesiak, M. et al. (2010) Stem Cells and Dev. 19: 669–677. |
| 2. Bühring et al. (2007) Ann. NY Acad. Sci. 1106: 262–271. |
| 3. Battula, V. L. et al. (2007) Differentiation 75: 279–291. |
| 4. Rozemuller, H. et al. (2010) Stem Cells Dev. 19: 1911-1921. |
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