| Description |
| The MSC Research Tool Box – MSCA-1 (W8B2), based on the antibody clone W8B2, was developed for the standardized and optimized enrichment and expansion of human mesenchymal stromal cells (MSCs) from bone marrow aspirates. The tool box contains all necessary reagents for the magnetic isolation of MSCA-1+ cells, including Anti-MSCA-1 (W8B2) MicroBeads for magnetic labeling and FcR Blocking Reagent to block non-specific labeling via Fc receptors. Furthermore, the MSC Research Tool Box – MSCA-1 (W8B2) contains NH Expansion Medium, a specialized medium for the expansion of preselected MSCs, and CytoMix – MSC, a cytokine cocktail designed specifically for the optimal and reproducible expansion of MSCs. The Anti-MSCA-1 (W8B2) MicroBeads recognize the mesenchymal stromal cell antigen-1 (MSCA-1), a so far unknown antigen. MSCA-1 was shown to be restricted to MSCs in the CD271bright population in bone marrow.1 These CD271brightCD45dim MSCs have a much higher clonogenic capacity compared to the CD271+CD45+ fraction in bone marrow. Therefore, MSCA-1 is a highly specific marker to isolate MSCs with a high proliferative potential from bone marrow. MSCA-1 expression was not found on placenta-derived MSCs after culture.2 |
| Applications |
| Positive selection and expansion of MSCs with a high proliferative capacity from bone marrow aspirates. |
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| Figure |
| MSCA-1 (W8B2)+ cells were isolated from human bone marrow mononuclear cells (BM-MNCs) using the MSC Research Tool Box –MSCA-1 (W8B2), two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC. |
| Before separation |
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| MSCA-1– cells |
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| MSCA-1+ cells |
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