| Description |
| Anti-Fibroblast MicroBeads have been developed for the positive selection or depletion of human fibroblasts using a fibroblast-specific antigen.1 This antigen is also known to be expressed on marrow stromal cells (MSCs) and putative mesenchymal progenitor cells (MPCs) in the bone marrow.2–4 |
| Applications |
| Applications of the Anti-Fibroblast MicroBeads include the isolation of MSCs from bone marrow aspirate or the removal of fibroblastic cells from bone marrow cultures or other primary tissue cultures. The depletion of fibroblast cells can be an effective step in the establishment of primary tumor cell lines in order to prevent fibroblastic overgrowth within the tissue culture. |
| |
| Figure 1 |
| Fibroblast antigen–positive cells were isolated from bone marrow mononuclear cells (BM-MNCs) using the Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD271 (LNGFR)-PE and CD45-FITC. (Courtesy of Dr. Elena Jones, Leeds, UK.) |
| BM-MNCs before separation |
 |
| Fibroblast antigen– cells |
 |
| Fibroblast antigen+ cells |
 |
|
|
