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| CD271 (LNGFR) MicroBead Kits |
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| Overview |
CD271 MicroBead Kits can be used for:
- Positive selection or depletion of cells expressing human CD271 (LNGFR).
- Positive selection of MSCs from human bone marrow or other tissues, including lipoaspirate.
- Positive selection of MSCs from bone marrow of monkey, goat, dog, pig, and sheep.
- Positive selection of neural cells, including neural crest cells, motor neurons, and Schwann cells.
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| Details |
Background information
CD271, also known as LNGFR (low-affinity nerve growth factor receptor) or p75NTR, belongs to the low-affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily.
CD271 is a well-known marker on mesenchymal stem cells, also known as mesencymal stromal cells, (MSCs) from bone marrow aspirate1–4 or lipoaspirate5. After separation colony-forming unit fibroblast (CFU-F) activity was found only in the CD271+ cell fraction, and not in the CD271– population6-8. Isolated CD271+ cells have a higher proliferative capacity in comparison to MSCs isolated by plastic adherence6,8,9. Secretion of growth factors was significantly higher in the separated CD271+ MSCs7.
CD271 (LNGFR) was initially described to be expressed on cells of the central and peripheral nervous system nervous system and was suggested to be involved in the development, survival, and differentiation of neural cells10.
CD271 (LNGFR) is expressed on
- Mesenchymal stem/stromal cells (MSCs)6,11,12
- Follicular dendritic cells13
- Mesenchymal cells involved in mesenchymal-epithelial interactions14
- autonomic and sensory neurons15
- oligodendrocytes16
- astrocytes17
- Schwann cells18,19
- Neural crest stem cells20
The CD271 antibody also recognizes MSCs in bone marrow from monkey, goat, dog, pig, and sheep21. |
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| Figure 1 |
| Enrichment of CD271+ mesenchymal stromal cells (MSCs) from bone marrow mononuclear cells (BM-MNCs) using the CD271 MicroBead Kit (APC) (A nd B) or the CD271 MicroBead Kit (PE) (C and D), an MS Column, and a MiniMACS Separator. |
| A: BM-MNCs before separation |
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| B: CD271+ cells |
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| C: BM-MNCs before separation |
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| D: CD271+ cells |
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| Details |
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| References |
| 1. Jones et al. (2004) (Abstract) 4th Annual Conference on Mesenchymal and Nonhematopoietic stem cells. |
| 2. Jones, E. et al. (2004) 29 ASH Abstract (2337) |
| 3. Jones et al. (2006) Cytometry B Clin. Cytom. 70(6): 391-399. |
| 4. Jones et al. (2007) MACS&more 11-1: 22–25. |
| 5. Meyerrose et al. (2007) Stem Cells 25: 220–227. |
| 6. Quirici et al. (2002) Exp. Hematol. 30: 783–791. |
| 7. Kuci et al. (2010) Haematologica 95: 651–659. |
| 8. Jarocha et al. (2008) Folia Histochem. Cytobiol. 46: 307–314. |
| 9. Poloni et al. (2009) Cytotherapy 11: 153–162. |
| 10. Thomson, T. M. et al. (1988) Exp. Cell Res. 174 (2): 533–539. |
| 11. Caneva et al. (1995) Blood Cells Mol. Dis. 21: 73–85. |
| 12. Jones et al. (2002) Arthritis & Rheumatism 46: 3349–3360. |
| 13. Pezzati et al. (1992) Immunology 76: 485–490. |
| 14. Huber and Chao (1995) Dev. Biol. 167: 227–238. |
| 15. Kashiba, H. et al. (1995) Brain Res. Mol. Brain Res. 30 (1): 158–164. |
| 16. Casaccia-Bonnefil, P. et al. (1996) Nature 383 (6602): 716–719. |
| 17. Rudge, J. S. et al. (1994) Eur J. Neurosci. 6 (5): 693–705. |
| 18. DiStefano, P. S. and Johnson, E. M. Jr. (1988) Proc. Natl. Acad. Sci. USA 85 (1): 270–274. |
| 19. Vroemen, M. and Weidner, N. (2003) J. Neuroscience Methods 124 (2): 135–143. |
| 20. Chalazonitis, A. et al. (1998) Dev Biol. 204 (2): 385–406. |
| 21. Rozemuller et al. (2010) Stem Cells Dev. 19: 1911-1921. |
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