CD271 (LNGFR) MicroBead Kit
Description The CD271 (LNGFR) MicroBead Kit is an indirect labeling system for the immunomagnetic isolation of CD271 (LNGFR)+ cells. The kit includes all necessary reagents for fluorescent labeling (CD271 (LNGFR)-PE or -APC) and indirect magnetic labeling (Anti-PE or -APC MicroBeads) of CD271 (LNGFR)+ cells, as well as FcR Blocking Reagent for the prevention of unspecific labeling via Fc receptors. CD271, also known as LNGFR (low affinity nerve growth factor receptor) or p75NTR, belongs to the low affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily. CD271 (LNGFR) was initially described to be expressed on cells of the nervous system and was suggested to be involved in the development, survival, and differentiation of neural cells.1 CD271 (LNGFR) can be found in the central and peripheral nervous system on autonomic and sensory neurons2 as well as on glial cells, including oligodendrocytes3, astrocytes4, Schwann cells5,6, and neural crest precursors7. CD271 (LNGFR) is a well-known marker for the enrichment of nonhematopoietic stem cells from bone marrow aspirates8–11, termed marrow stromal cells (MSCs). After separation, colony forming unit fibroblast (CFU-F) activity was found only in the CD271+ cell fraction, by comparison to the CD271– population, and these cells were further shown to have a 10- to 100-fold higher proliferative capacity in comparison to MSCs isolated by plastic adherence.12 Furthermore, CD271 has been described as a specific marker for MSCs from lipoaspirate.13 Applications Positive selection or depletion of cells expressing human CD271 (LNGFR). Positive selection of MSCs from the bone marrow or other tissues, including lipoaspirate. Positive selection of neural cells, including neural crest cells, motor neurons, and Schwann cells. Columns MS, LS, XS, or autoMACS™ Columns. Further information CD271_poster [PDF; 235,9 KB] Isolation of CD271 (LNGFR)+ MSCs/ADSCs from human lipoaspirate [PDF; 457,7 KB]		Figure 1 Enrichment of CD271+ marrow stromal cells (MSCs) from bone marrow mononuclear cells (BM-MNCs) using the CD271 MicroBead Kit (APC) (A nd B) or the CD271 MicroBead Kit (PE) (C and D), an MS Column, and a MiniMACS Separator. A: BM-MNCs before separation with the CD271 MicroBead Kit (APC) B: CD271+ cells C: Before separation with the CD271 MicroBead Kit (PE) D: CD271+ cells