| Description |
The Lineage Cell Depletion Kit is an indirect magnetic labeling system for the depletion of mature hematopoietic cells such as T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, erythroid cells, and their committed precursors from bone marrow aspirate, cord blood, or mobilized leukapheresis product. The depletion of lineage-committed cells results in the enrichment of untouched stem and progenitor cells.
For depletion, cells are first labeled with a cocktail of biotin-conjugated antibodies targeting a panel of so-called “lineage” antigens: CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a (glycophorin A), and then labeled with Anti-Biotin MicroBeads. |
| Applications |
- Enrichment of lineage-negative cells from human bone marrow aspirate, cord blood, and leukapheresis product.
- Enrichment of hematopoietic in vitro colony-forming cells (CFC, CFU-GM, BFU-E, and CFU-Mk) and primitive long-term culture-initiating cells.
- Enrichment of nonhematopoietic in vitro colony-forming cells (CFU-Fs) from bone marrow aspirate.
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| Figure A) |
| Isolation of untouched lineage-negative cells from bone marrow mononuclear cells (BM-MNCs) using the Lineage Cell Depletion Kit and an LS Column. Cells were fluorescently stained with CD34-FITC. |
| BM-MNCs before separation |
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| Lineage+ fraction |
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| CD34+ cells in the lineage cell–depleted fraction. |
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