| Description |
| The MSC Research Tool Box – CD271 (LNGFR) has been developed for the standardized and optimized enrichment and expansion of human mesenchymal stromal cells (MSCs) from bone marrow aspirates. The tool box includes all necessary reagents for fluorescent labeling (CD271 (LNGFR)-PE or -APC) and indirect magnetic labeling (Anti‑PE or -APC MicroBeads) of CD271 (LNGFR)+ cells, as well as FcR Blocking Reagent to block unspecific labeling via Fc receptors. Furthermore, the MSC Research Tool Box – CD271 (LNGFR) contains NH Expansion Medium, a specialized medium for the expansion of MSCs, and CytoMix – MSC, a cytokine cocktail designed specifically for the optimal and reproducible expansion of preselected MSCs. |
| Applications |
CD271, also known as low-affinity nerve growth factor receptor (LNGFR), is a well-known marker for the isolation of MSCs directly from bone marrow aspirate.1–5 After separation, colony forming unit fibroblast (CFU-F) activity was found only in the CD271+ cell fraction, by comparison to the CD271– population, and these cells were further shown to have a 10- to 100-fold higher proliferative capacity in comparison to MSCs isolated by plastic adherence.5 Furthermore, CD271 has been described as a specific marker for MSCs from lipoaspirate.6
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| Figure 1 |
| Isolation and expansion of bone marrow-derived CD271+ mesenchymal stromal cells using the MSC Research Tool Box – CD271 (LNGFR). CD271+ cells were isolated using CD271 (LNGFR)-APC and Anti-APC MicroBeads (A and B), or CD271 (LNGFR)-PE and Anti-PE MicroBeads (C and D). FcR Blocking Reagent was used to prevent unspecific labeling. CD271+ cells were then expanded in NH Expansion Medium supplemented with CytoMix – CD271 (LNGFR) (E). |
| A: BM-MNCs before separation |
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| B: CD271+ cells |
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| C: BM-MSCs before separation |
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| D: CD271+ cells |
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| E: CD271+ cells after expansion |
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| Figure 2 |
| Isolation, expansion and further differentiation of CD271+ cells using the MSC Research Tool Box – CD271 (LNGFR) and NH Differentiation Media. |
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