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| MSC Research Tool Box – CD271 (LNGFR) |
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| Overview |
| The MSC Research Tool Box – CD271 (LNGFR) has been developed for standardized and optimized enrichment and expansion of human mesenchymal stromal cells (MSCs) from bone marrow, lipoaspirate and other tissues. |
| Details |
Background information
CD271, also known as LNGFR (low-affinity nerve growth factor receptor) or p75NTR, belongs to the low-affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily.
CD271 is a well-known marker on mesenchymal stem cells, also known as mesencymal stromal cells, (MSCs) from bone marrow aspirate1–4 or lipoaspirate5. After separation colony-forming unit fibroblast (CFU-F) activity was found only in the CD271+ cell fraction, and not in the CD271– population6-8. Isolated CD271+ cells have a higher proliferative capacity in comparison to MSCs isolated by plastic adherence6,8,9. Secretion of growth factors was significantly higher in the separated CD271+ MSCs7.
CD271 (LNGFR) was initially described to be expressed on cells of the central and peripheral nervous system nervous system and was suggested to be involved in the development, survival, and differentiation of neural cells10.
CD271 (LNGFR) is expressed on - Mesenchymal stem/stromal cells (MSCs)6,11,12
- Follicular dendritic cells13
- Mesenchymal cells involved in mesenchymal-epithelial interactions14
- autonomic and sensory neurons15
- oligodendrocytes16
- astrocytes17
- Schwann cells18,19
- Neural crest precursors20
- Neural crest stem cells20
The CD271 antibody also recognizes MSCs in bone marrow from monkey, goat, dog, pig, and sheep21.
Detailed procedure
The tool box includes all necessary reagents for fluorescent labeling (CD271 (LNGFR)-PE or -APC) and indirect magnetic labeling (Anti-PE or Anti-APC MicroBeads) of CD271 (LNGFR)+ cells, as well as FcR Blocking Reagent to block non-specific labeling via Fc receptors, NH Expansion Medium, a specialized medium for the expansion of MSCs, and CytoMix – MSC, a cytokine cocktail designed specifically for the optimal and reproducible expansion of pre-selected MSCs. |
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| Figure 1 |
| CD271+ cells were isolated from human bone marrow using CD271 (LNGFR)-APC and Anti-APC MicroBeads (A and B), or CD271 (LNGFR)-PE and Anti-PE MicroBeads (C and D). FcR Blocking Reagent was used to prevent unspecific labeling. CD271+ cells were then expanded in NH Expansion Medium supplemented with CytoMix – CD271 (LNGFR) (E). |
| A: BM-MNCs before separation |
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| B: CD271+ cells |
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| C: BM-MNCs before separation |
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| D: CD271+ cells |
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| E: CD271+ cells after expansion |
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| Favorites / Prices |
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| Details |
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| Products |
| MSC Research Tool Box – CD271 (APC), human |
- for 109 total cells
Download datasheet
130-092-291
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| MSC Research Tool Box – CD271 (PE), human |
- for 109 total cells
Download datasheet
130-092-867
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| References |
| 1. Jones et al. (2004) (Abstract) 4th Annual Conference on Mesenchymal and Nonhematopoietic stem cells. |
| 2. Jones et al. (2004) (Abstract) 29. ASH (2337). |
| 3. Jones et al. (2006) Cytometry B Clin. Cytom. 70: 391–399. |
| 4. Jones et al. (2007) MACS&more 11-1: 22–25. |
| 5. Meyerrose et al. (2007) Stem Cells 25: 220–227. |
| 6. Quirici et al. (2002) Exp. Hematol. 30: 783–791. |
| 7. Kuci et al. (2010) Haematologica 95: 651–659. |
| 8. Jarocha et al. (2008) Folia Histochem. Cytobiol. 46: 307–314. |
| 9. Poloni et al. (2009) Cytotherapy 11: 153–162. |
| 10. Thomson, T. M. et al. (1988) Exp. Cell Res. 174 (2): 533–539. |
| 11. Caneva et al. (1995) Blood Cells Mol. Dis. 21: 73–85. |
| 12. Jones et al. (2002) Arthritis & Rheumatism 46: 3349–3360. |
| 13. Pezzati et al. (1992) Immunology 76: 485–490. |
| 14. Huber and Chao (1995) Dev. Biol. 167: 227–238. |
| 15. Kashiba, H. et al. (1995) Brain Res. Mol. Brain Res. 30 (1): 158–164. |
| 16. Casaccia-Bonnefil, P. et al. (1996) Nature 383 (6602): 716–719. |
| 17. Rudge, J. S. et al. (1994) Eur J. Neurosci. 6 (5): 693–705. |
| 18. DiStefano, P. S. and Johnson, E. M. Jr. (1988) Proc. Natl. Acad. Sci. USA 85 (1): 270–274. |
| 19. Vroemen, M. and Weidner, N. (2003) J. Neuroscience Methods 124 (2): 135–143. |
| 20. Chalazonitis, A. et al. (1998) Dev Biol. 204 (2): 385–406. |
| 21. Rozemuller et al. (2010) Stem Cells Dev. 19: 1911-1921. |
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