| Description |
The CD117 MicroBead Kit has been developed for the separation of human cells based on their expression of the CD117 antigen. The kit also includes FcR Blocking Reagent to avoid unspecific labeling of cells via Fc receptors. The MicroBead-conjugated CD117 antibody (clone AC126) does not interfere with stem cell factor (SCF) binding; the epitope is identical or in close proximity to that of the CD117 antibody 104D2, but different to the epitope recognized by clone A3C6E2. CD117 (also known as c-kit or stem cell factor receptor) is a 145 kDa cell surface glycoprotein with tyrosine kinase activity. The molecule is suggested to be involved in signaling, activation, and proliferation of cells. CD117 is expressed on approximately 1–3% of peripheral blood mononuclear cells and cord blood cells, and on up to 10% of bone marrow cells where approximately 25% of the CD117+ cells were found to coexpress CD133 and CD34. In addition, CD117 is expressed on basophils, myeloid dendritic cells, TCRα/β+ T cells, CD19+ B cells, and CD56+ NK cells1, as well as on mast cells, melanocytes, and acute myeloid leukemic blasts2.
For quality control staining of CD117-separated cells, the use of CD117 (A3C6E2) is receommended due to its recognition of a different epitope to that of clone AC126. It should also be noted that clone A3C6E2 potentially interferes with the SCF binding site on CD117. |
| Applications |
| Beside their hematopoietic differentiation potential, CD117+ cells isolated from human bone marrow were also reported to have at least osteoblast differentiation potential.3 Therefore, it is of special interest to analyze CD117+ cells for their nonhematopoietic differentiation potential in vivo and in vitro. Fetal-derived stem cells have also been isolated from amniotic fluid cultures according to the expression of CD117 and show a multipotent ability to differentiate into cell types of each embryonic germ layer.4 |
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