| Description |
| The CD133 MicroBead Kit, formerly termed CD133 Cell Isolation Kit, is a magnetic labeling system designed for the positive selection of CD133+ cells. It allows the single-step isolation of nonhematopoietic and early hematopoietic progenitors and stem cells. CD133+ cells can be isolated from peripheral blood, cord blood, bone marrow, leukapheresis product, as well as single-cell suspensions of solid tissues and ESC-derived cells1. Isolated from hematopoietic sources, CD133+ cells can become adherent and are reported to become CD133-negative during culture.2,3 These adherent cells can then in turn give rise to nonadherent CD133+ cells that are able to differentiate to both hematopoietic and nonhematopoietic cell types.4 The CD133 MicroBead Kit contains FcR Blocking Reagent to avoid unspecific labeling of cells via Fc receptors, and MACS® MicroBeads conjugated to a monoclonal mouse anti-human CD133 antibody (clone AC133) that recognizes epitope CD133/1. For quality control staining of CD133-separated cells, the use of CD133/2 (293C3)-PE or -APC is recommended. |
| Applications |
| CD133+ cells are used in basic stem cell research, stem cell evaluation, research in stem cell plasticity, stem cell expansion, angiogenesis, research into cellular therapy and tissue engineering or regeneration, and research in hematological malignancies as well as in tumor-initiating cells/cancer stem cells. For information on clinical products click here. |
| Clone | Isotype |
| AC133 | Mouse IgG1 |
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| Figure 1 |
| Isolation of CD133+ cells from human PBMCs using the CD133 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. |
| PBMCs before separation |
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| Isolated CD133+ cells |
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| Figure 2 |
Isolated CD133+ cells during cultivation.
[Cells were stained with CD133/1 (AC133)-PE.] |
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| Figure 3 |
| Isolation of CD133+ cells using the CD133 MicroBead Kit. |
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