Agilent CGH Services
From samples to visualized CGH microarray data As certified Agilent Service provider, Miltenyi Biotec offers high-quality CGH microarray services based on Agilent 60-mer oligonucleotide microarrays. Flexible CGH microarray services Miltenyi Biotec offers comprehensive CGH services with Agilent‘s CGH microarray platform — from DNA extraction to data analysis, from whole genome to custom. Moreover, in combination with the Agilent Microarray Service genomic copy-number changes can be correlated with gene expression changes. Service provider for reliable data The service team processes Agilent CGH Microarrays and provides an extensive report including a record of all experimental steps and data analyses. The CGH Microarray Services are strictly confidential. Experimental results and digital data remain intellectual property of the customer. Detection of of chromosomal changes Comparative genomic hybridization (CGH) is used to study copy-number variations in any region of the genome. Agilent's CGH microarray platform allows the identification and characterization of various chromosomal imbalances in cancer and genetic disorders. This will help researchers to identify new oncogenes, tumor suppressor genes, drug targets, biomarkers, etc. Agilent Genome CGH Microarrays Genome-wide coverage on a single microarray Agilent CGH microarrays are high-resolution tools for genome-wide DNA variation profiling. Comprehensive probe coverage spans both coding and non-coding regions. High resolution and sensitivity Probe design and selection have been carefully optimized and validated for maximal sensitivity and specificity, allowing reproducible detection of genomic lesions including single-copy and homozygous deletions, as well as variable amplicons. Agilent CGH 4×44K Microarray — for deletion analysis The microarray consists of about 43,000 probes with an average probe spatial resolution of 35 kb and an emphasis on the most commonly studied genomic coding regions and cancer-related genes. At least one probe represents well-characterized genes while cancer-relevant genes are each represented by two or more probes. Agilent CGH 244A Microarray — for higher precision Consisting of about 235,000 probes with an average probe spatial resolution of 6.4 kb, this microarray has an emphasis not only on well-known genes but also promotors, miRNAs, and telomeric regions. Thus, it enables even better precision in mapping chromosomal breakpoints and identifying novel microvariations. Custom Agilent High Definition-CGH Microarrays High-resolution tiling capability Custom High-Definition CGH Microarrays can be designed from a database of about eight million in silico-validated CGH probes, spaced at 400 bp on average, to focus on specific genomic regions. Customizable content Choose your region of interest to design a customized microarray tailored to your research needs, leveraging pre-designed CGH probes that cover both exonic, intronic, and intergenic regions of the genome. Further information Agilent_CGH_Microarray_Flyer [PDF; 255 KB] CGH Microarray Order Confirmation non-US [PDF; 158,6 KB] CGH Microarray Order Confirmation_US [PDF; 95,2 KB]		Figure 1 Figure 2 Genome View Agilent´s CGH Analytics Software visualizes the CGH microarray data: Detected aberrations are marked green for deletions and red for amplifications. For example, in chromosome 9, framed by a box, the deletion in the p arm is indicated in green. Genomic DNA derived from Jurkat cells was hybridized versus male genomic DNA as a reference to the Agilent Human Genome CGH Microarray 44B. Figure 3 Chromosome View An example of a chromosomal aberration in Jurkat cells is shown for chromosome 9. Every dot represents one probe. The blue-shaded area indicates the deletion located in the region p21.3. Figure 4 Gene View The Gene View zooms into the deleted region of chromosome 9 and identifies genes that are located in this region.

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