| Description |
The CD66abce MicroBead Kit is an indirect magnetic labeling system for enrichment or depletion of CD66+ neutrophils from peripheral blood.
The human CD66 antigens CD66a, b, c, e belong to the family of carcinoembryonic antigen–related cell adhesion molecules (CEACAM). They are involved in a wide variety of cellular processes, including cell adhesion, activation, proliferation, differentiation, survival, and apoptosis. CD66a (also known as CEACAM1, BGP, NCA-160, and C-CAM) is expressed by certain epithelial, endothelial, and hematopoietic cells. Among leukocytes, it is highly expressed on neutrophils. Upon activation, cell surface expression of CD66a is upregulated on various leukocytes, including granulocytes, T cells, and NK cell subsets. CD66b (CEACAM8, NCA-95, CGM6) is also expressed on granulocytes, CD66c (CEACAM6, NCA-50/90)is found on on granulocytes and epithelial cells. CD66e (CEA, CEACAM5) is over-expressed in certain tumors of epithelial origin. |
| Applications |
| CD66abce MicroBeads are used for the isolation or depletion of human neutrophils from lysed human peripheral blood. Neutrophils play a prominent role in the innate immune response through their phagocytic activity. They are abundant in peripheral blood but almost absent in healthy tissue. Upon infection, large numbers of neutrophils are recruited to the sites of inflammation. |
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| Figure 1 |
| Isolation of CD66abce+ cells from human peripheral blood leukocytes, using the CD66abce MicroBead Kit, an MS Column, and an appropriate separator. Cells are fluorescently stained with Anti-Biotin-PE and CD15-FITC. |
| 1: Before separation |
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| 2: Isolated CD66abce+ cells |
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