| Overview |
| The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs. |
| Details |
Detailed separation procedure
Non-basophils (i.e. T cells, NK cells, B cells, monocytes, dendritic cells, erythroid cells, platelets, neutrophils, and eosinophils) are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure basophils is achieved by depletion of the magnetically labeled cells.
Downstream applications
Isolated basophils have been used for phenotypical characterization3, for histamine, leukotriene, and cytokine1, 4, 5, 6 release assays, for molecular studies1, 2, 5, protein analysis4, 5, and other applications. |
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| Figure 1 |
| Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c+ cells or CD123brightCD303– cells because CD123 is expressed at high levels on both basophils and CD303+ plasmacytoid dendritic cells. |
| Before separation |
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| B |
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| Isolated basophils |
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| D |
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| Figure 2 |
| Basophils isolated with MACS® Technology using the Basophil Isolation Kit II. (Courtesy of Dr. Karin Haisch, Borstel, Germany.) |
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