CD16 MicroBeads
Description CD16 MicroBeads are typically used for the isolation of highly pure eosinophils2 by magnetic depletion of neutrophils from granulocyte-enriched cell fractions from peripheral blood (fig. 2). CD16 MicroBeads can also be used for the positive selection of human neutrophils from peripheral blood or various tissues such as bone marrow and nasal tissues. The CD16 antigen, which is a low affinity Fc receptor for aggregated IgG, is expressed on neutrophils, NK cells, and macrophages. Therefore, CD16 MicroBeads have also been used to deplete NK cells. Applications Eosinophils isolated with CD16 MicroBeads are used for the functional analysis of surface molecules1,3 or for studies on signal transduction7, chemotaxis1,6, cytokine expression3,5, degranulation4, and apoptosis7. Columns LD, CS, D, or autoMACS™ Columns.		Figure 1 Isolation of eosinophils from peripheral blood using CD16 MicroBeads. Figure 2 Eosinophils isolated using CD16 MicroBeads and MACS® Technology. (Courtesy of Dr. Hans-Uwe Simon, Davos, Switzerland.)