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- Unique: simultaneous separation of eosinophil and neutrophil granulocytes
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| Overview |
| CD16 MicroBeads have been developed for the isolation of eosinophils by depletion of neutrophils from granulocyte-enriched cell fractions from peripheral blood. Alternatively, neutrophils can be isolated by positive selection. Peripheral blood and various tissues (e.g., bone marrow, nasal tissue) are suitable cell sources. |
| Details |
Background information
The CD16 antigen, which is a low-affinity Fc receptor for aggregated IgG, is expressed on neutrophils, NK cells, and macrophages. CD16 MicroBeads have also been used to deplete NK cells.
Downstream applications
Eosinophils isolated with CD16 MicroBeads are used for the functional analysis of surface molecules1,3 or for studies on signal transduction7, chemotaxis1,6, cytokine expression3,5, degranulation4, and apoptosis7. |
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| Figure 1 |
| Isolation of eosinophils from peripheral blood using CD16 MicroBeads. |
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| Figure 2 |
| Eosinophils isolated using CD16 MicroBeads and MACS® Technology. (Courtesy of Dr. Hans-Uwe Simon, Davos, Switzerland.) |
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| Details |
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| References |
| 1. Blom et al. (1995) J. Immunol. Methods 178: 183-193. |
| 2. Hansel et al. (1991) J. Immunol. Methods 145: 105-110. |
| 3. Levi-Schaffer et al. (1995) Blood 85: 2579-2586. |
| 4. Simon et al. (1994) J. Immunol. 153: 364-377. |
| 5. Sriramarao et al. (1996) J. Immunol. 157: 4672-4680. |
| 6. Motegi and Kita (1998) J. Immunol. 161: 4340-4346. |
| 7. Yousefi et al. (1996) J. Exp. Med. 183: 1407-1414. |
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