Whole Blood CD15 MicroBeads
Description Whole Blood CD15 MicroBeads were developed for automated positive selection of CD15+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD15 MicroBeads to be subsequently separated with the autoMACS™ Pro Separator or the autoMACS Separator. Whole Blood CD15 MicroBeads recognize the carbohydrate structure 3-fucosyl-N-acetyllactosamine (3-FAL), also designated Lewis X. The CD15 antigen is expressed on neutrophils and eosinophils but not on basophils and lymphocytes. Applications Whole Blood MicroBeads and the autoMACS™ Pro Separator or the autoMACS Separator allow fast sample processing under standardized conditions. This combination further allows easy and safe handling of hazardous samples. The purified CD15+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2,3. For more information on MACS® Whole Blood MicroBeads click here. Separation Program autoMACS™ Pro Separator: Posselwb (0.25–15 mL sample volume). autoMACS Separator: Posseld2 (0.25–3 mL sample volume).		Figure 1 CD15+ cells were separated from whole blood using Whole Blood CD15 MicroBeads and the autoMACS™ Separator with program Posseld2. Cells were fluorescently stained with CD15-FITC and CD45-PE. Figure 2 Cell isolation by using Whole Blood MicroBeads and the autoMACS™ Pro Separator or the autoMACS Separator.